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Hfr strains of Escherichia coli were obtained by integrative suppression of a dnaA(Ts) mutation by the Inc P-1 plasmid RP1 without prior creation of an unnatural homology between the plasmid and the E. coli chromosome. Unmodified RP1 mobilized the polarized transfer of the chromosome in a counterclock-wise direction from a distinct origin between 81 min (pyrE) and 82 min (dnaA) with pyrE as a leading marker. Inheritance of RP1-Hfr chromosomal and antibiotic resistance genes was due to recombination with the recipient chromosome, as shown by the need for a functional recA system. The acquisition of temperature resistance and donor ability was accompanied by the disappearance of free plasmid when the selection pressure for integration was maintained (growth at 41 degrees C); the loss of temperature resistance and donor ability was accompanied by the reappearance of autonomous RP1 when the selection pressure was removed (growth at 30 degrees C).
R R Martin, and
C L Thorlton, and
L Unger
October 1983,
Genetika,
R R Martin, and
C L Thorlton, and
L Unger
January 1977,
Molecular & general genetics : MGG,
R R Martin, and
C L Thorlton, and
L Unger
February 1982,
Antibiotiki,
R R Martin, and
C L Thorlton, and
L Unger
September 1969,
Genetics,
R R Martin, and
C L Thorlton, and
L Unger
November 1976,
Journal of molecular biology,
R R Martin, and
C L Thorlton, and
L Unger
July 1979,
Plasmid,
R R Martin, and
C L Thorlton, and
L Unger
April 1986,
The Journal of biological chemistry,
R R Martin, and
C L Thorlton, and
L Unger
January 1965,
Annales medicinae experimentalis et biologiae Fenniae,
R R Martin, and
C L Thorlton, and
L Unger
October 1967,
Proceedings of the National Academy of Sciences of the United States of America,
R R Martin, and
C L Thorlton, and
L Unger
September 1986,
Molecular & general genetics : MGG,
