Biomaterial Database

The amino acid sequence of the histidine binding protein of Salmonella typhimurium. 1981

R W Hogg

The amino acid sequence of the histidine binding protein of Salmonella typhimurium was determined by automated sequence analysis of reduced and S-pyridylethylated histidine binding protein and fragments derived by chemical and enzymatic cleavage of the native protein. The fragments were the products of cleavage at methionine residues by cyanogen bromide, cleavage at tryptophan residues by 2-nitrophenylsulfenyl-3-methyl-3-bromo-3H-indole (BrNps-skatole), limited enzymatic digestion at arginine residues, and enzymatic digestion at Glu-X bonds by the Staphylococcus aureus V8 protease. The sequence of the COOH-terminal residues was determined using bovine carboxypeptidases A and B and amino acid analysis. The histidine binding protein was found to contain 238 amino acid residues and to have a molecular weight of 26,104 calculated from sequence.

UI	MeSH Term	Description	Entries
D010446	Peptide Fragments	Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.	Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D010447	Peptide Hydrolases	Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.	Peptidase,Peptidases,Peptide Hydrolase,Protease,Proteases,Proteinase,Proteinases,Proteolytic Enzyme,Proteolytic Enzymes,Esteroproteases,Enzyme, Proteolytic,Hydrolase, Peptide
D003488	Cyanogen Bromide	Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes.	Bromide, Cyanogen
D006639	Histidine	An essential amino acid that is required for the production of HISTAMINE.	Histidine, L-isomer,L-Histidine,Histidine, L isomer,L-isomer Histidine
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D012486	Salmonella typhimurium	A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.	Salmonella typhimurium LT2