-transparent.png){kind=logo}
A glycoprotein immunologically related to plasma cold-insoluble globulin (CIG) and fetal skin fibroblast fibronectin has been purified from second-trimester human amniotic fluid. This protein (amniotic fluid fibronectin) migrated more slowly than CIG on sodium dodecyl sulfate gel electrophoresis and showed greater polydispersity which could result, at least in part, from heterogeneity in glycosylation. Cloned human amniotic fluid epithelioid and fibroblastic cells synthesized and secreted a protein with similar properties into the culture medium. Fibronectin was shown to be associated with the pericellular and extracellular matrix of cultured amniotic fluid cells by immunofluorescence, lactoperoxidase-catalyzed iodination, and labeling with ferritin-conjugated antibodies. The kinetics of secretion of the protein were consistent with its role as a matrix protein. We anticipate that amniotic fluid fibronectin will prove to be the same protein which elsewhere in the body is incorporated into connective tissues and basement membranes. Amniotic fluid could, therefore, serve as a convenient source of in vivo synthesized fibronectin for biological and structural studies.
E Crouch, and
G Balian, and
K Holbrook, and
D Duksin, and
P Bornstein
March 1991,
Ginecologia y obstetricia de Mexico,
E Crouch, and
G Balian, and
K Holbrook, and
D Duksin, and
P Bornstein
May 1979,
The Journal of biological chemistry,
E Crouch, and
G Balian, and
K Holbrook, and
D Duksin, and
P Bornstein
December 1978,
Biochemistry,
E Crouch, and
G Balian, and
K Holbrook, and
D Duksin, and
P Bornstein
January 1980,
Renal physiology,
E Crouch, and
G Balian, and
K Holbrook, and
D Duksin, and
P Bornstein
July 1983,
The American journal of physiology,
E Crouch, and
G Balian, and
K Holbrook, and
D Duksin, and
P Bornstein
January 1988,
American journal of perinatology,
E Crouch, and
G Balian, and
K Holbrook, and
D Duksin, and
P Bornstein
April 2013,
Research in veterinary science,
E Crouch, and
G Balian, and
K Holbrook, and
D Duksin, and
P Bornstein
February 1970,
British medical journal,
E Crouch, and
G Balian, and
K Holbrook, and
D Duksin, and
P Bornstein
November 1984,
Endocrinology,
E Crouch, and
G Balian, and
K Holbrook, and
D Duksin, and
P Bornstein
January 1985,
Intensive care medicine,
