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The method described permits an exact and rapid determination of aldosterone in urine and plasma. The reliability of the method is based on the separation of aldosterone from contaminating steroids by thin-layer chromatography. The mobile phase used was: cyclohexane--ethyl acetate (20:80). The steroids were extracted by dichloromethane. Plasma was extracted directly, and urine after hydrolysis with sulfuric acid (pH = 1). Recovery before radioimmunological analysis of aldosterone was 54.8 +/- 7.2 (S.D.)%(N = 40) for urine samples, and 39.1 +/- 4.4% (n = 60) for plasma samples. The coefficient of variation for multiple determinations of aldosterone was for urine 8.2% for low (n = 10) and 7.8% (n = 10). The sensitivity of the determination of aldosterone was for urine 0.04 microgram per 24-h volume (n = 10) and for plasma 4.4 ng per 100 ml (n = 10). The method avoids pitfalls due to the cross-reaction of anti-aldosterone serum with other materials.
K Herkner, and
P Nowotny, and
W Waldhäusl
June 1981,
Casopis lekaru ceskych,
K Herkner, and
P Nowotny, and
W Waldhäusl
March 1974,
Zeitschrift fur klinische Chemie und klinische Biochemie,
K Herkner, and
P Nowotny, and
W Waldhäusl
January 1979,
Annales d'endocrinologie,
K Herkner, and
P Nowotny, and
W Waldhäusl
June 1978,
Radioisotopes,
K Herkner, and
P Nowotny, and
W Waldhäusl
November 1975,
Deutsche medizinische Wochenschrift (1946),
K Herkner, and
P Nowotny, and
W Waldhäusl
August 1974,
Klinische Wochenschrift,
K Herkner, and
P Nowotny, and
W Waldhäusl
September 1979,
Medizinische Klinik,
K Herkner, and
P Nowotny, and
W Waldhäusl
March 1982,
Zhonghua yi xue za zhi,
K Herkner, and
P Nowotny, and
W Waldhäusl
April 1982,
Horumon to rinsho. Clinical endocrinology,
K Herkner, and
P Nowotny, and
W Waldhäusl
June 1979,
Nihon Jinzo Gakkai shi,
