Biomaterial Database

Routine direct injection gas--liquid chromatographic procedure for the analysis of volatile halogenated anaesthetics in whole blood using a new external injection port. 1978

P E Reid, and D E Brooks, and Y C Pang, and R Muelchen

This communication describes the design and construction of a new external injection port for the direct gas--liquid chromatographic analysis of volatile compounds in whole blood. Aliquots (4--40 microliter) of EDTA anti-coagulated blood containing the volatile compound and a weighed quantity of the internal standard, isobutanol, were injected into the disposable glass wool filter of the carrier gas stream directly onto the chromatographic column. Typical data are presented from chromatography performed with dual 6 ft. x 2 mm I.D. glass columns containing Chromosorb 101 programmed from 110--180 degrees at 6 degrees/min and the external injection port maintained at 180 degrees. The method eliminated the problems usually associated with direct injection methods and permitted the accurate analysis of halothane, methoxyflurane, diethyl ether and ethanol over the approximate range 1--100 mg%. Using this analytical procedure the distribution of halothane between the cells and plasma of human blood at 4 degrees was found to be 2.0 +/- 0.2.

UI	MeSH Term	Description	Entries
D007267	Injections	Introduction of substances into the body using a needle and syringe.	Injectables,Injectable,Injection
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D002849	Chromatography, Gas	Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.	Chromatography, Gas-Liquid,Gas Chromatography,Chromatographies, Gas,Chromatographies, Gas-Liquid,Chromatography, Gas Liquid,Gas Chromatographies,Gas-Liquid Chromatographies,Gas-Liquid Chromatography
D006221	Halothane	A nonflammable, halogenated, hydrocarbon anesthetic that provides relatively rapid induction with little or no excitement. Analgesia may not be adequate. NITROUS OXIDE is often given concomitantly. Because halothane may not produce sufficient muscle relaxation, supplemental neuromuscular blocking agents may be required. (From AMA Drug Evaluations Annual, 1994, p178)	1,1,1-Trifluoro-2-Chloro-2-Bromoethane,Fluothane,Ftorotan,Narcotan
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000777	Anesthetics	Agents capable of inducing a total or partial loss of sensation, especially tactile sensation and pain. They may act to induce general ANESTHESIA, in which an unconscious state is achieved, or may act locally to induce numbness or lack of sensation at a targeted site.	Anesthetic,Anesthetic Agents,Anesthetic Drugs,Anesthetic Effect,Anesthetic Effects,Agents, Anesthetic,Drugs, Anesthetic,Effect, Anesthetic,Effects, Anesthetic
D014835	Volatilization	A phase transition from liquid state to gas state, which is affected by Raoult's law. It can be accomplished by fractional distillation.	Vaporization,Volatility