Biomaterial Database

IgG antibody to Giardia lamblia detected by enzyme-linked immunosorbent assay. 1981

P D Smith, and F D Gillin, and W R Brown, and T E Nash

A sensitive, reproducible enzyme-linked immunosorbent assay using unfixed, intact Giardia lamblia trophozoites as antigen is described. Employing this assay, we demonstrated that 81% of 59 symptomatic giardiasis patients and 12% of 17 uninfected control subjects had circulating IgG antibodies to G. lamblia. Eleven out of 15 patients tested serially had detectable antibody from 2 wk to 15 mo after therapy for acute infection. The prevalence of antibody in 197 unselected individuals in the Washington, D.C. area was 14%. Specificity of the antibodies was demonstrated by the ability of live G. lamblia trophozoites, but not Entamoeba histolytica or Trichomonas vaginalis trophozoites, to absorb the antibody activity. High titers of antibody were detected in 5 out of 7 patients with recurrent infection. The enzyme-linked immunosorbent assay described here may help to further characterize the antibody response to infection with G. lamblia.

UI	MeSH Term	Description	Entries
D007074	Immunoglobulin G	The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.	Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007163	Immunosorbent Techniques	Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.	Immunoadsorbent Techniques,Immunoadsorbent Technics,Immunosorbent Technics,Immunoadsorbent Technic,Immunoadsorbent Technique,Immunosorbent Technic,Immunosorbent Technique,Technic, Immunoadsorbent,Technic, Immunosorbent,Technics, Immunoadsorbent,Technics, Immunosorbent,Technique, Immunoadsorbent,Technique, Immunosorbent,Techniques, Immunoadsorbent,Techniques, Immunosorbent
D008297	Male		Males
D002648	Child	A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL.	Children
D002675	Child, Preschool	A child between the ages of 2 and 5.	Children, Preschool,Preschool Child,Preschool Children
D004219	District of Columbia	A federal area located between Maryland and Virginia on the Potomac river; it is coextensive with Washington, D.C., which is the capital of the United States.	Washington, D.C.,Washington, DC
D005872	Giardia	A genus of flagellate intestinal EUKARYOTES parasitic in various vertebrates, including humans. Characteristics include the presence of four pairs of flagella arising from a complicated system of axonemes and cysts that are ellipsoidal to ovoidal in shape.	Lamblia,Giardias,Lamblias
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia