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Methyl benzimidazol-2-yl-carbamate (MBC), at a concentration of 100 microM, has a pronounced effect on the growth of Saccharomyces cerevisiae, resulting in the accumulation of cells as large doublets. We have determined a specific execution point for the effect of MBC on the yeast cell cycle, and have shown that this execution point is between the cycle events of spindle pole body duplication and spindle pole body separation. An ultrastructural examination of the MBC-treated cells revealed the absence of cytoplasmic and spindle microtubules. MBC treatment also produced an altered spindle pole body morphology, causing the disappearance of the outer component. Nuclear size was also markedly increased in the MBC-induced doublet cells, although the septa were completely absent from these doublet cells. It is proposed that MBC inhibits microtubule polymerization, rather than causing the depolymerization of stable microtubules.
R A Quinlan, and
C I Pogson, and
K Gull
September 1982,
Molecular and cellular biology,
R A Quinlan, and
C I Pogson, and
K Gull
January 1987,
Journal of toxicology and environmental health,
R A Quinlan, and
C I Pogson, and
K Gull
January 1981,
Mutation research,
R A Quinlan, and
C I Pogson, and
K Gull
November 1990,
Mutation research,
R A Quinlan, and
C I Pogson, and
K Gull
January 1978,
Oncology,
R A Quinlan, and
C I Pogson, and
K Gull
September 1982,
Molecular and cellular biology,
R A Quinlan, and
C I Pogson, and
K Gull
April 1984,
Nucleic acids research,
R A Quinlan, and
C I Pogson, and
K Gull
August 1998,
Cell structure and function,
R A Quinlan, and
C I Pogson, and
K Gull
May 1976,
Journal of general microbiology,
R A Quinlan, and
C I Pogson, and
K Gull
February 1977,
Experimental cell research,
