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An improved preparation of antibody-coated polystyrene beads for sandwich enzyme immunoassay of human thyroid-stimulating hormone (TSH) was described. Rabbit anti-TSH IgG was purified by eluting at pH 2.5 from a TSH-Sepharose column, diluted 3 or 9 fold with normal rabbit IgG and used for coating polystyrene beads by physical adsorption. In a sandwich enzyme immunoassay of TSH using rabbit (anti-TSH) Fab'-beta-D-galactosidase conjugate, beta-D-galactosidase activities specifically bound to thus prepared polystyrene beads in the presence of TSH was 2.8-6.3 fold higher than those bound to polystyrene beads coated with anti-TSH IgG before purification. A similar effect was observed when guinea pig anti-pork insulin IgG, rabbit (anti-human IgE) IgG and goat (anti-human IgE) IgG were treated at pH 2.5. This improvement may be based on a conformational change of Fc in IgG molecule which was caused by the treatment at pH 2.5. Other sandwich immunoassays such as fluoro- and radio-immunoassays may also be improved in the same way.
E Ishikawa, and
Y Hamaguchi, and
M Imagawa, and
M Inada, and
H Imura, and
N Nakazawa, and
H Ogawa
December 2006,
Journal of immunological methods,
E Ishikawa, and
Y Hamaguchi, and
M Imagawa, and
M Inada, and
H Imura, and
N Nakazawa, and
H Ogawa
January 1985,
Annales de l'Institut Pasteur. Immunologie,
E Ishikawa, and
Y Hamaguchi, and
M Imagawa, and
M Inada, and
H Imura, and
N Nakazawa, and
H Ogawa
February 1988,
Endocrinologia japonica,
E Ishikawa, and
Y Hamaguchi, and
M Imagawa, and
M Inada, and
H Imura, and
N Nakazawa, and
H Ogawa
January 1984,
Journal of biochemistry,
E Ishikawa, and
Y Hamaguchi, and
M Imagawa, and
M Inada, and
H Imura, and
N Nakazawa, and
H Ogawa
January 1986,
Journal of immunoassay,
E Ishikawa, and
Y Hamaguchi, and
M Imagawa, and
M Inada, and
H Imura, and
N Nakazawa, and
H Ogawa
January 1981,
Journal of clinical microbiology,
E Ishikawa, and
Y Hamaguchi, and
M Imagawa, and
M Inada, and
H Imura, and
N Nakazawa, and
H Ogawa
February 2010,
Analytical chemistry,
E Ishikawa, and
Y Hamaguchi, and
M Imagawa, and
M Inada, and
H Imura, and
N Nakazawa, and
H Ogawa
August 1979,
Clinical chemistry,
E Ishikawa, and
Y Hamaguchi, and
M Imagawa, and
M Inada, and
H Imura, and
N Nakazawa, and
H Ogawa
April 1989,
Clinical chemistry,
E Ishikawa, and
Y Hamaguchi, and
M Imagawa, and
M Inada, and
H Imura, and
N Nakazawa, and
H Ogawa
January 1999,
Biotechnology progress,
