-transparent.png){kind=logo}
A synthetic (Syn) medium was developed for growth of Micropolyspora faeni and Thermoactinomyces candidus, and optimum conditions for culture filtrate antigen production were found to be 3 days for T. candidus and 9 to 12 days for M. faeni. Appearance of proteolytic activity in the culture fluid supernatant coincided with a decrease in precipitating antigen content, protein content, and number of proteins on polyacrylamide gel electrophoresis (PAGE), probably as a result of proteolysis. The results suggest that protein content, number protein bands on PAGE, and proteolytic activity are predictive of precipitating antigen content. Antigens prepared in Syn medium were compared to those produced by the double-dialysis procedure and found to contain adequate amounts of antigenic material of sufficient quality to warrant clinical studies of their usefulness in the diagnosis of hypersensitivity pneumonitis.
M W Treuhaft, and
R C Roberts, and
C Hackbarth, and
J J Marx
February 1977,
Infection and immunity,
M W Treuhaft, and
R C Roberts, and
C Hackbarth, and
J J Marx
November 1981,
Infection and immunity,
M W Treuhaft, and
R C Roberts, and
C Hackbarth, and
J J Marx
January 1979,
International archives of allergy and applied immunology,
M W Treuhaft, and
R C Roberts, and
C Hackbarth, and
J J Marx
January 1974,
Annals of the New York Academy of Sciences,
M W Treuhaft, and
R C Roberts, and
C Hackbarth, and
J J Marx
July 1989,
Allergy,
M W Treuhaft, and
R C Roberts, and
C Hackbarth, and
J J Marx
February 1984,
Mycopathologia,
M W Treuhaft, and
R C Roberts, and
C Hackbarth, and
J J Marx
April 1978,
The Journal of allergy and clinical immunology,
M W Treuhaft, and
R C Roberts, and
C Hackbarth, and
J J Marx
April 1982,
Clinical chemistry,
M W Treuhaft, and
R C Roberts, and
C Hackbarth, and
J J Marx
April 1978,
The Journal of allergy and clinical immunology,
M W Treuhaft, and
R C Roberts, and
C Hackbarth, and
J J Marx
July 1978,
Canadian journal of microbiology,
