[ED-20 epoxy resin as an embedding medium for biological objects for electron microscopy study]. 1981

V I Grechishnikov

A method of embedding of biological objects for electron microscopy is described. A solution of epoxide resin ED-20 with a plastifier, dibutyl phthalate, and a solidifier, standard polyethylene polyamine, comprising 8% and 12% of the total mass of resin, was added into capsules into which blocks of tissues to be examined that had been pre-impregnated in increasing resin-in-acetone solution series were placed. Polymerization occurred under conventional conditions at room temperature for 24 hours or at 37 degrees C for 12 hours.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D003584 Cytological Techniques Methods used to study CELLS. Cytologic Technics,Cytological Technic,Cytological Technics,Cytological Technique,Technic, Cytological,Technics, Cytological,Technique, Cytological,Techniques, Cytological,Cytologic Technic,Technic, Cytologic,Technics, Cytologic
D004853 Epoxy Resins Polymeric resins derived from OXIRANES and characterized by strength and thermosetting properties. Epoxy resins are often used as dental materials. Epoxy Resin,Resin, Epoxy,Resins, Epoxy

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