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The mode of replication of the L double-stranded RNA (dsRNA) present in virus-like particles in Saccharomyces cerevisiae was examined by density transfer experiments. After transfer to light medium, significant amounts of fully heavy dsRNA persisted over a number of cell doublings. In addition, very little material of hybrid density was ever formed, and the accumulation of fully light material began as early as 0.5 doubling after transfer to light medium. Our results are compatible with a conservative mode of replication or with a semiconservative mode of replication carried out by a small portion of the total dsRNA population. In additional experiments the synthesis of dsRNA relative to the cell cycle was studied. This was done by determining the ratio of short-term to long-term radioactive label in size-separated cell fractions of a prelabeled exponential culture. The ratio of short-term to long-term label remained constant for all fractions, implying that dsRNA is synthesized throughout the cell cycle, increasing through the cell cycle at an exponential rate.
A M Newman, and
S G Elliott, and
C S McLaughlin, and
P A Sutherland, and
R C Warner
June 1986,
Proceedings of the National Academy of Sciences of the United States of America,
A M Newman, and
S G Elliott, and
C S McLaughlin, and
P A Sutherland, and
R C Warner
January 1986,
Basic life sciences,
A M Newman, and
S G Elliott, and
C S McLaughlin, and
P A Sutherland, and
R C Warner
May 1986,
Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology,
A M Newman, and
S G Elliott, and
C S McLaughlin, and
P A Sutherland, and
R C Warner
January 1978,
Microbios,
A M Newman, and
S G Elliott, and
C S McLaughlin, and
P A Sutherland, and
R C Warner
March 1974,
The Journal of general virology,
A M Newman, and
S G Elliott, and
C S McLaughlin, and
P A Sutherland, and
R C Warner
March 1996,
Microbiological reviews,
A M Newman, and
S G Elliott, and
C S McLaughlin, and
P A Sutherland, and
R C Warner
March 1986,
Journal of virology,
A M Newman, and
S G Elliott, and
C S McLaughlin, and
P A Sutherland, and
R C Warner
January 1986,
Basic life sciences,
A M Newman, and
S G Elliott, and
C S McLaughlin, and
P A Sutherland, and
R C Warner
September 1990,
Molecular and cellular biology,
A M Newman, and
S G Elliott, and
C S McLaughlin, and
P A Sutherland, and
R C Warner
January 1992,
Annual review of microbiology,
