Klebsiella serotyping by counter-current immunoelectrophoresis. 1978

J M Palfreyman

The development of a Klebsiella serotyping method by counter-current immunoelectrophoresis (CIE) is described. Antisera were prepared against the capsular antigens of 72 type strains and tested for the specificity and strength of their precipitin reactions with antigens from homologous and heterologous serotypes. All antisera produced strong reactions with their homologous antigen: when diluted to titre 63 were highly specific, 3 cross-reacted strongly and 6 weakly with one other antigen. Pools of antisera for screening purposes were constructed on the basis of common crossreactions: component serotypes of each pool could be detected strongly and specifically. The technique is simple to perform, fairly rapid, and economical in the use of antisera. Results can be read easily and quickly and the intensity of cross-reactions compared directly. The technique appears to be more specific and is less time consuming than the Quellung method, but further assessment of its efficacy in typing routine clinical cultures is necessary.

UI MeSH Term Description Entries
D007122 Immunoelectrophoresis A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D007709 Klebsiella A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria whose organisms arrange singly, in pairs, or short chains. This genus is commonly found in the intestinal tract and is an opportunistic pathogen that can give rise to bacteremia, pneumonia, urinary tract and several other types of human infection.
D003378 Counterimmunoelectrophoresis Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow. Electrosyneresis,Immunoelectroosmophoresis,Immunoelectrophoresis, Countercurrent,Immunoelectrophoresis, Crossover,Countercurrent Immunoelectrophoresis,Crossover Immunoelectrophoresis
D012703 Serotyping Process of determining and distinguishing species of bacteria or viruses based on antigens they share. Serotypings

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