Biomaterial Database

Immunocytochemical localization of vitamin D-dependent calcium binding protein in mammalian nephron. 1981

W B Rhoten, and S Christakos

Immunoreactive calcium binding protein (iCaBP) has been localized in the rat nephron using the unlabeled antibody peroxidase-antiperoxidase immunocytochemical technique. Kidneys from normal young adult, vitamin D-deficient, and 12 day old rats were prepared by either freeze-substitution or 1% glutaraldehyde-Bouin's fixation. CaBP was localized with rabbit antiserum to chicken vitamin D-dependent intestinal CaBP. iCaBP was found specifically in the distal convoluted tubules (DCT); however, not all cells of the DCT were positive. In adult nephrons, a few scattered cells apparently belonging to the collecting tubules were positive. In the neonatal nephrons, there was also localization of iCaBP to the thick limb of Henle, suggesting a difference in the regulation of intracellular calcium during maturation. Proximal tubules, renal corpuscles, macula densa, and thin limbs of Henle gave no specific localization of iCaBP. These results present for the first time the localization of iCaBP in the mammalian nephron both in the neonate and in the young adult.

UI	MeSH Term	Description	Entries
D007422	Intestines	The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.	Intestine
D007668	Kidney	Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.	Kidneys
D007672	Kidney Cortex	The outer zone of the KIDNEY, beneath the capsule, consisting of KIDNEY GLOMERULUS; KIDNEY TUBULES, DISTAL; and KIDNEY TUBULES, PROXIMAL.	Cortex, Kidney
D008297	Male		Males
D009399	Nephrons	The functional units of the kidney, consisting of the glomerulus and the attached tubule.	Nephron
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D002135	Calcium-Binding Proteins	Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.	Calcium Binding Protein,Calcium-Binding Protein,Calcium Binding Proteins,Binding Protein, Calcium,Binding Proteins, Calcium,Protein, Calcium Binding,Protein, Calcium-Binding
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D006652	Histological Techniques	Methods of preparing tissue for examination and study of the origin, structure, function, or pathology.	Histologic Technic,Histologic Technics,Histologic Technique,Histologic Techniques,Histological Technics,Technic, Histologic,Technics, Histologic,Technique, Histologic,Techniques, Histologic,Histological Technic,Histological Technique,Technic, Histological,Technics, Histological,Technique, Histological,Techniques, Histological
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia