Biomaterial Database

Substrate specificity of CTP-synthetase from E. coli. 1981

K H Scheit, and H J Linke

The substrate specificity of CTP-synthetase from E. coli was investigated by means of UTP analogs. This study revealed that the three main structural elements of the UTP molecule were important for the substrate specificity of the enzyme. CTP-synthetase seems to possess an absolute requirement for the beta-D-ribose 5-triphosphate part in UTP. Substitutents in 5-position of UTP, exceeding the size of a tritium atom abolish substrate function.

UI	MeSH Term	Description	Entries
D008025	Ligases	A class of enzymes that catalyze the formation of a bond between two substrate molecules, coupled with the hydrolysis of a pyrophosphate bond in ATP or a similar energy donor. (Dorland, 28th ed) EC 6.	Ligase,Synthetases,Synthetase
D003570	Cytidine Triphosphate	Cytidine 5'-(tetrahydrogen triphosphate). A cytosine nucleotide containing three phosphate groups esterified to the sugar moiety.	CTP,CRPPP,Magnesium CTP,Mg CTP,Triphosphate, Cytidine
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D013329	Structure-Activity Relationship	The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.	Relationship, Structure-Activity,Relationships, Structure-Activity,Structure Activity Relationship,Structure-Activity Relationships
D013379	Substrate Specificity	A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.	Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D014500	Uracil Nucleotides	A group of pyrimidine NUCLEOTIDES which contain URACIL.	Uridine Phosphates,Uridine Nucleotides,Nucleotides, Uracil,Nucleotides, Uridine,Phosphates, Uridine
D014544	Uridine Triphosphate	Uridine 5'-(tetrahydrogen triphosphate). A uracil nucleotide containing three phosphate groups esterified to the sugar moiety.	UTP,Magnesium UTP,Magnesium Uridine Triphosphate,Mg-UTP,Mg UTP,Triphosphate, Magnesium Uridine,Triphosphate, Uridine,UTP, Magnesium
D019731	Carbon-Nitrogen Ligases	Enzymes that catalyze the joining of two molecules by the formation of a carbon-nitrogen bond. EC 6.3.	Carbon Nitrogen Ligases,Ligases, Carbon-Nitrogen