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We have developed a sandwich-type enzyme immunoassay for human chorionic gonadotropin (hCG), in which antibody Fab'-beta-D-galactosidase complex and an antibody-immobilized silicone rubber solid phase were used. Despite the fact this assay system crossreacted about 40% with human luteinizing hormone (LH) that contains an immunologically very similar subunit to that of hCG, it proved to be highly sensitive with hCG measurable at levels as low as 0.3 mIU per assay tube. Using 25 microliter of urine sample, hCG levels in serum (10-1000 mlU/ml) or in urine (3-300 mlU/ml) could be determine with the same degree of precision as in radioimmunoassay without sample interference with the assay. The coefficients of variation in within-run, and between-run were 9.2-13.3%, and 4.2-18.8%, respectively. Values obtained with enzyme immunoassay correlate well with those of radioimmunoassay (r = 0.961, slope = 1.129, y-intercept = 3.7 mlU/ml for 35 serum samples) and hemagglutination assay (r = 0.954, slope = 0.951, y-intercept = 1.8 mlU/ml for 88 urine samples).
T Sekiya, and
Y Furuhashi, and
S Goto, and
S Kaseki, and
Y Tomoda, and
K Kato
December 1986,
Scandinavian journal of clinical and laboratory investigation,
T Sekiya, and
Y Furuhashi, and
S Goto, and
S Kaseki, and
Y Tomoda, and
K Kato
January 1985,
Annales de l'Institut Pasteur. Immunologie,
T Sekiya, and
Y Furuhashi, and
S Goto, and
S Kaseki, and
Y Tomoda, and
K Kato
December 1980,
Endocrinologia japonica,
T Sekiya, and
Y Furuhashi, and
S Goto, and
S Kaseki, and
Y Tomoda, and
K Kato
October 1978,
Endocrinologia japonica,
T Sekiya, and
Y Furuhashi, and
S Goto, and
S Kaseki, and
Y Tomoda, and
K Kato
April 1981,
Journal of biochemistry,
T Sekiya, and
Y Furuhashi, and
S Goto, and
S Kaseki, and
Y Tomoda, and
K Kato
March 1990,
Asia-Oceania journal of obstetrics and gynaecology,
T Sekiya, and
Y Furuhashi, and
S Goto, and
S Kaseki, and
Y Tomoda, and
K Kato
June 1977,
The Indian journal of medical research,
T Sekiya, and
Y Furuhashi, and
S Goto, and
S Kaseki, and
Y Tomoda, and
K Kato
July 1981,
Acta obstetrica et gynaecologica Japonica,
T Sekiya, and
Y Furuhashi, and
S Goto, and
S Kaseki, and
Y Tomoda, and
K Kato
November 1988,
Annals of clinical biochemistry,
T Sekiya, and
Y Furuhashi, and
S Goto, and
S Kaseki, and
Y Tomoda, and
K Kato
June 1985,
Journal of immunological methods,
