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The development of the monoclonal antibody YC5/45 HLK (YC5/HLK) against a 5HT-bovine seroalbumin immunogen and its application for immunocytochemistry is described. The YC5/HLK antibody is the product of a rat x rat hybrid myeloma, producing a heavy chain and two light chains. In hemagglutination tests, the antibody cross-reacts to entirety with dopamine, serotonin, and tryptamine at high concentrations. The serotonin-albumin conjugate is 20,000 times more effective in displacing the binding antibody, while albumin itself goes unrecognized by the antibody. In fixed preparations of brain tissue, immunofluorescence is observed only in neurons known to contain serotonin, while no reaction is observed in dopamine-rich neurons. All immunofluorescence is extinguished by the use of agents that inhibit the biosynthesis of 5HT, but not of the catecholamines.
A Consolazione, and
C Milstein, and
B Wright, and
A C Cuello
October 1983,
Journal of immunological methods,
A Consolazione, and
C Milstein, and
B Wright, and
A C Cuello
January 1995,
Human antibodies and hybridomas,
A Consolazione, and
C Milstein, and
B Wright, and
A C Cuello
January 1985,
Histochemistry,
A Consolazione, and
C Milstein, and
B Wright, and
A C Cuello
January 1991,
Neuroscience,
A Consolazione, and
C Milstein, and
B Wright, and
A C Cuello
May 1992,
Zhonghua fu chan ke za zhi,
A Consolazione, and
C Milstein, and
B Wright, and
A C Cuello
March 1990,
American journal of clinical pathology,
A Consolazione, and
C Milstein, and
B Wright, and
A C Cuello
December 2021,
Cells,
A Consolazione, and
C Milstein, and
B Wright, and
A C Cuello
January 1985,
Haematology and blood transfusion,
A Consolazione, and
C Milstein, and
B Wright, and
A C Cuello
January 1988,
Acta cytologica,
A Consolazione, and
C Milstein, and
B Wright, and
A C Cuello
January 1993,
Anticancer research,
