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A double-antibody, solid-phase enzyme-linked immunosorbent assay was developed for detection of candida antigen(s) in serum and body fluids. The assay detected mannan purified from Candida albicans cells with a sensitivity of 1 ng/ml in buffer, but it was 1,000-fold less sensitive in pooled human serum. Because antibody appeared to be the major blocking factor in serum, a rapid and efficient heat-extraction method for recovering mannan from antibody-containing serum was devised. The assay detected antigenemia within 24 hr in all of 30 lethally infected rats, and antigenemia persisted until their deaths. Eight of 15 patients with documented or suspected candidiasis had antigenemia at levels ranging from 5 to greater than 1,000 ng/ml.
M A Lew, and
G R Siber, and
D M Donahue, and
F Maiorca
May 1983,
The Journal of laboratory and clinical medicine,
M A Lew, and
G R Siber, and
D M Donahue, and
F Maiorca
January 1987,
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
M A Lew, and
G R Siber, and
D M Donahue, and
F Maiorca
July 1987,
Taiwan yi xue hui za zhi. Journal of the Formosan Medical Association,
M A Lew, and
G R Siber, and
D M Donahue, and
F Maiorca
January 1988,
Jikken dobutsu. Experimental animals,
M A Lew, and
G R Siber, and
D M Donahue, and
F Maiorca
February 1989,
Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology,
M A Lew, and
G R Siber, and
D M Donahue, and
F Maiorca
April 1987,
Laboratory animal science,
M A Lew, and
G R Siber, and
D M Donahue, and
F Maiorca
May 1983,
Journal of clinical microbiology,
M A Lew, and
G R Siber, and
D M Donahue, and
F Maiorca
April 1984,
The Indian journal of medical research,
M A Lew, and
G R Siber, and
D M Donahue, and
F Maiorca
February 1985,
Rinsho byori. The Japanese journal of clinical pathology,
M A Lew, and
G R Siber, and
D M Donahue, and
F Maiorca
October 1992,
Australian veterinary journal,
