Biomaterial Database

Age-related decline in the synthesis of glycosaminoglycans by cultured human fibroblasts (WI-38). 1978

D O Schachtschabel, and J Wever

A gradual decline in the synthesis of glycosaminoglycans, as evidenced by reduced rates of incorporation of [35S]-sulfate and [14C]-glucosamine into total cellular and extracellular glycosaminoglycans, occurred during the last 4 to 5 passages (1 : 2 splits) of WI-38 cultures before phase out. While labelling of cellular glycosaminoglycans by both radioactive precursors was reduced to about the same extent, a relatively greater decline in [35S]-sulfate than in [14C]-glucosamine incorporation into extracellular glycosaminoglycans was observed during the last passages. These changes in glycosaminoglycan metabolism are interpreted as an expression of cellular aging and a function of glycosaminoglycans in growth regulation (and possibly in the process of cellular senescence) is discussed.

UI	MeSH Term	Description	Entries
D011849	Radioactive Tracers	Radioactive substances added in minute amounts to the reacting elements or compounds in a chemical process and traced through the process by appropriate detection methods, e.g., Geiger counter. Compounds containing tracers are often said to be tagged or labeled. (Hawley's Condensed Chemical Dictionary, 12th ed)	Radioactive Tracer,Radionuclide Tracer,Radionuclide Tracers,Tracer, Radioactive,Tracer, Radionuclide,Tracers, Radioactive,Tracers, Radionuclide
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D006025	Glycosaminoglycans	Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine (see ACETYLGLUCOSAMINE) or N-acetylgalactosamine (see ACETYLGALACTOSAMINE).	Glycosaminoglycan,Mucopolysaccharides
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor