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Glomerular basement membranes (GBM) were isolated and subjected to enzymatic degradation with the protease trypsin (Serva), chymotrypsin (Serva), papain (Sigma), pepsin (Serva) and collagenase (Worthington) as well as a lysosomal preparation from glass adherent rat blood and peritoneal exudate cells. Split products were characterized by immunoelectrophoresis and cellulose acetate electrophoresis. Urine was obtained from healthy rats and rats with Masugi's experimental glomerulonephritis, dialyzed and concentrated and applied on immunoelectrophoresis, using anti-GBM antibody from rabbit. Urinary GBM split products from healthy and nephrotic rats showed two precipitation lines like digestion products obtained after chymotrypsin degradation. This finding was supported by characterizing individual antigenic degradation products obtained after inhibition of GBM degradation by the lysosomal preparation with ethylenediaminetetraacetic acid and Trasylol alone and in combination, as well as with o-phenanthrolin. It is concluded that GBM-antigens excreted into urine indicate limited digestion of GBM by chymotrypsin-type protease.
G Lubec, and
O Förster, and
H Coradello, and
E Maxa, and
A Pollak
January 1979,
Biology of the neonate,
G Lubec, and
O Förster, and
H Coradello, and
E Maxa, and
A Pollak
January 1987,
Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie,
G Lubec, and
O Förster, and
H Coradello, and
E Maxa, and
A Pollak
January 1987,
Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie,
G Lubec, and
O Förster, and
H Coradello, and
E Maxa, and
A Pollak
November 1967,
Clinica chimica acta; international journal of clinical chemistry,
G Lubec, and
O Förster, and
H Coradello, and
E Maxa, and
A Pollak
January 1977,
Contributions to nephrology,
G Lubec, and
O Förster, and
H Coradello, and
E Maxa, and
A Pollak
January 1980,
Renal physiology,
G Lubec, and
O Förster, and
H Coradello, and
E Maxa, and
A Pollak
December 1979,
European journal of biochemistry,
G Lubec, and
O Förster, and
H Coradello, and
E Maxa, and
A Pollak
January 1976,
Comparative biochemistry and physiology. B, Comparative biochemistry,
G Lubec, and
O Förster, and
H Coradello, and
E Maxa, and
A Pollak
January 1975,
Annales de biologie clinique,
G Lubec, and
O Förster, and
H Coradello, and
E Maxa, and
A Pollak
September 1990,
Journal of clinical & laboratory immunology,
