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Washed platelets, contaminated with less than 0.20% plasma factor XI, were examined for the presence of factor XI antigen and activity. These platelets contained a factor-XI-like coagulant activity (0.67 +/- 0.11 U/10(11) platelets) that remained constant after successive washes. By means of indirect immunofluorescence, a monospecific antibody to factor XI showed specific staining of both normal platelets and platelets from patients deficient in plasma factor XI. Radiolabeled Triton extracts of washed platelets and labeled purified factor XI solutions were analyzed for factor XI antigen by Staph A immunoprecipitation analysis using antibody to purified plasma factor XI followed by SDS gel electrophoresis. On unreduced gels, the platelet material ran as a single band having an apparent molecular weight of 220,000 daltons, whereas purified plasma factor XI gave a single band at 160,000 daltons. On reduced gels, the platelet material analyzed as a single band at 52,000 daltons, whereas purified factor XI gave a single band of 80,000 daltons. Analysis of a partially purified factor XI preparation from platelets by immunoelectrophoresis revealed that the platelet preparation displayed a slightly lower cathodal electrophoretic mobility at pH 8.6 than did plasma factor XI and yet appeared to possess complete antigenic identity with plasma factor XI. These results indicate that platelets possess a form of factor XI that exists as a disulfide-linked 52,000-dalton tetramer in contrast to the plasma form that circulates as a 80,000-dalton disulfide-linked dimer.
G P Tuszynski, and
S J Bevacqua, and
A H Schmaier, and
R W Colman, and
P N Walsh
March 1977,
British journal of haematology,
G P Tuszynski, and
S J Bevacqua, and
A H Schmaier, and
R W Colman, and
P N Walsh
August 1976,
Blood,
G P Tuszynski, and
S J Bevacqua, and
A H Schmaier, and
R W Colman, and
P N Walsh
May 1979,
The Journal of clinical investigation,
G P Tuszynski, and
S J Bevacqua, and
A H Schmaier, and
R W Colman, and
P N Walsh
November 1999,
Blood,
G P Tuszynski, and
S J Bevacqua, and
A H Schmaier, and
R W Colman, and
P N Walsh
October 1985,
Thrombosis research,
G P Tuszynski, and
S J Bevacqua, and
A H Schmaier, and
R W Colman, and
P N Walsh
March 1985,
Blood,
G P Tuszynski, and
S J Bevacqua, and
A H Schmaier, and
R W Colman, and
P N Walsh
March 2021,
American journal of physiology. Cell physiology,
G P Tuszynski, and
S J Bevacqua, and
A H Schmaier, and
R W Colman, and
P N Walsh
October 2004,
Journal of thrombosis and haemostasis : JTH,
G P Tuszynski, and
S J Bevacqua, and
A H Schmaier, and
R W Colman, and
P N Walsh
July 1986,
Biochemistry,
G P Tuszynski, and
S J Bevacqua, and
A H Schmaier, and
R W Colman, and
P N Walsh
January 1977,
Haemostasis,
