-transparent.png){kind=logo}
Human CRP and the CRPs of other species bind Ca2+ ion. After binding of the divalent cation, CRP binds all phosphate monoesters with a stochiometry of one mole per mole of CRP subunit. Replacement of the phosphate monoester group by other acidic groups or by conversion to a phosphodiester markedly diminishes or abolishes the ability to bind. Phosphorylcholine is bound by CRP with much higher affinity than other phosphate monoesters speaking for a second binding site with specificity for the positively charged trimethylammonium group. The distance separating the phosphate from the positively charged group may be relevant. Protein that has been coupled with phosphorylcholine or phosphorylethanolamine is able to precipitate with CRP. Several natural substances including pneumococcal-C polysaccharide which react with CRP have been found to contain phosphorylcholine. In addition CRP has another binding site accounting for its ability to react with depyruvylated type 4 pneumococcal polysaccharide which does not contain phosphate or choline.
E C Gotschlich, and
T Y Liu, and
E Oliveira
March 1991,
Journal of immunology (Baltimore, Md. : 1950),
E C Gotschlich, and
T Y Liu, and
E Oliveira
February 1994,
Biochemical Society transactions,
E C Gotschlich, and
T Y Liu, and
E Oliveira
January 1985,
International review of experimental pathology,
E C Gotschlich, and
T Y Liu, and
E Oliveira
January 1987,
FEBS letters,
E C Gotschlich, and
T Y Liu, and
E Oliveira
October 1982,
Infection and immunity,
E C Gotschlich, and
T Y Liu, and
E Oliveira
March 1986,
Journal of medical microbiology,
E C Gotschlich, and
T Y Liu, and
E Oliveira
June 1984,
The Journal of biological chemistry,
E C Gotschlich, and
T Y Liu, and
E Oliveira
March 1967,
Proceedings of the National Academy of Sciences of the United States of America,
E C Gotschlich, and
T Y Liu, and
E Oliveira
July 2006,
Glycoconjugate journal,
E C Gotschlich, and
T Y Liu, and
E Oliveira
February 1992,
The Journal of veterinary medical science,
