BIOMAT Database Logo BIOMAT Database Logo

Evaluation of a solid-phase immunofluorescence assay for detection of antibodies to Legionella pneumophila. 1982

T A Thompson, and H W Wilkinson

A semiautomated solid-phase immunofluorescence technique (FIAX) was compared with the standard indirect immunofluorescence assay (IFA) for the determination of antibody levels to Legionella pneumophila serogroup 1 in paired human serum samples. The FIAX method was in agreement with the IFA test for 91.8% of the serum pairs but gave evidence of a recent Legionella infection significantly fewer times than did the IFA. These results suggest that the FIAX technique may eventually be a useful alternative test for measuring Legionella antibodies. However, further study will be required to determine its efficacy in providing a serodiagnosis of legionellosis.

UI MeSH Term Description Entries
D007875 Legionella Gram-negative aerobic rods, isolated from surface water or thermally polluted lakes or streams. Member are pathogenic for man. Legionella pneumophila is the causative agent for LEGIONNAIRES' DISEASE.
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000907 Antibodies, Bacterial Immunoglobulins produced in a response to BACTERIAL ANTIGENS. Bacterial Antibodies
D000936 Antigen-Antibody Complex The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES. Immune Complex,Antigen-Antibody Complexes,Immune Complexes,Antigen Antibody Complex,Antigen Antibody Complexes,Complex, Antigen-Antibody,Complex, Immune,Complexes, Antigen-Antibody,Complexes, Immune
D001424 Bacterial Infections Infections by bacteria, general or unspecified. Bacterial Disease,Bacterial Infection,Infection, Bacterial,Infections, Bacterial,Bacterial Diseases

Related Publications

T A Thompson, and H W Wilkinson
Detection of antibodies to Legionella pneumophila in immune guinea pig serum by solid-phase immunofluorescence.
April 1981, Journal of clinical microbiology,
T A Thompson, and H W Wilkinson
Evaluation of Vircell enzyme-linked immunosorbent assay and indirect immunofluorescence assay for detection of antibodies against Legionella pneumophila.
March 2006, Clinical and vaccine immunology : CVI,
T A Thompson, and H W Wilkinson
Detection of antibodies to Legionella pneumophila serogroup 1 by indirect immunofluorescence and enzyme-linked immunosorbent assay.
May 1984, Bollettino dell'Istituto sieroterapico milanese,
T A Thompson, and H W Wilkinson
Detection of Legionella pneumophila-specific antibody by indirect immunofluorescence assay.
January 1992, Acta microbiologica Hungarica,
T A Thompson, and H W Wilkinson
[Enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Legionella pneumophila].
May 1986, Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
T A Thompson, and H W Wilkinson
Kinetic-dependent enzyme-linked immunosorbent assay for detection of antibodies to Legionella pneumophila.
December 1983, Journal of clinical microbiology,
T A Thompson, and H W Wilkinson
Indirect immunofluorescence antibodies to Legionella pneumophila: frequency in a rural community.
September 1980, Journal of clinical microbiology,
T A Thompson, and H W Wilkinson
Detection of antinuclear antibodies by indirect immunofluorescence and by solid phase assay.
October 2011, Autoimmunity reviews,
T A Thompson, and H W Wilkinson
Comparison and evaluation of four commercial kits relative to an in-house immunofluorescence test for detection of antibodies against Legionella pneumophila.
February 2008, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
T A Thompson, and H W Wilkinson
Validation of Legionella pneumophila indirect immunofluorescence assay with epidemic sera.
January 1981, Journal of clinical microbiology,
Copied contents to your clipboard!