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The optimization of a leukocyte stimulation microassay with carp (Cyprinus carpio L.) leukocytes is described. Leukocytes were isolated from the thymus, anterior kidney, spleen, mid-kidney and peripheral blood. Leukocyte cultures were stimulated with PHA-P, LPS (Escherichia coli 055: B5) PWM, ConA and PPD from Mycobacterium fortuitum. The optimum incubation temperature for leukocyte cultures differed 3.5 days for leukocyte cultures derived from lymphoid organs and 4.5 days for peripheral blood lymphocyte cultures. Leukocytes from various organ sources showed similar reactivity patterns to stimulation in vitro by different mitogens. The results of these mitogen stimulations did not present sufficient arguments in favour of compartmentation.
E W Liewes, and
R H Van Dam, and
M G Vos-Maas, and
R Bootsma
July 2009,
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP,
E W Liewes, and
R H Van Dam, and
M G Vos-Maas, and
R Bootsma
July 1998,
General and comparative endocrinology,
E W Liewes, and
R H Van Dam, and
M G Vos-Maas, and
R Bootsma
May 1973,
Experientia,
E W Liewes, and
R H Van Dam, and
M G Vos-Maas, and
R Bootsma
September 2006,
Fish & shellfish immunology,
E W Liewes, and
R H Van Dam, and
M G Vos-Maas, and
R Bootsma
January 1999,
Biochemistry and molecular biology international,
E W Liewes, and
R H Van Dam, and
M G Vos-Maas, and
R Bootsma
January 1979,
Acta biologica et medica Germanica,
E W Liewes, and
R H Van Dam, and
M G Vos-Maas, and
R Bootsma
January 2015,
BioMed research international,
E W Liewes, and
R H Van Dam, and
M G Vos-Maas, and
R Bootsma
January 1977,
Anatomischer Anzeiger,
E W Liewes, and
R H Van Dam, and
M G Vos-Maas, and
R Bootsma
May 1977,
Bulletin de l'Academie polonaise des sciences. Serie des sciences biologiques,
E W Liewes, and
R H Van Dam, and
M G Vos-Maas, and
R Bootsma
January 1974,
Journal de physiologie,
