Surface properties of Chlamydia psittaci. 1980

D W Vance, and T P Hatch

The surface properties of elementary bodies of Chlamydia psittaci were investigated by diethylaminoethyl-Sephadex chromatography, cytophoresis, partitioning in an aqueous polymer two-phase system, and hydrophobic interaction chromatography of the organism. The surface of C. psittaci was found to be hydrophobic and negatively charged at pH 7 and to have an isoelectric point of about pH 5. Reagents which block free carboxyl groups altered the surface charge of C. psittaci and caused the organism to agglomerate. The possible significance of hydrophobicity and surface charge on the ingestion of C. psittaci by host cells is discussed.

UI MeSH Term Description Entries
D007526 Isoelectric Point The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum. Isoelectric Points,Point, Isoelectric,Points, Isoelectric
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002691 Chlamydophila psittaci A genus of CHLAMYDOPHILA infecting primarily birds. It contains eight known serovars, some of which infect more than one type of host, including humans. Chlamydia psittaci
D002852 Chromatography, Ion Exchange Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins. Chromatography, Ion-Exchange,Ion-Exchange Chromatography,Chromatographies, Ion Exchange,Chromatographies, Ion-Exchange,Ion Exchange Chromatographies,Ion Exchange Chromatography,Ion-Exchange Chromatographies
D004586 Electrophoresis An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current. Electrophoreses
D004594 Electrophysiology The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
D005022 Ethyldimethylaminopropyl Carbodiimide Carbodiimide cross-linking reagent. 1-Ethyl-3-(3-Dimethylaminopropyl)Carbodiimide,3-(3-Dimethylaminopropyl)-1-Ethylcarbodiimide,EDAP-Carbodiimide,Carbodiimide, Ethyldimethylaminopropyl,EDAP Carbodiimide
D013499 Surface Properties Characteristics or attributes of the outer boundaries of objects, including molecules. Properties, Surface,Property, Surface,Surface Property

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