Biomaterial Database

Flexible sorting decision and droplet charging control electronic circuitry for flow cytometer-cell sorters. 1982

M J McCutcheon, and R G Miller

We describe sorting decision and droplet charging control electronic circuitry for a flow cytometer-cell sorter. The circuitry can handle up to five independent input signals and can sort in up to four directions. A sixth input channel is used to determine the presence or absence of an event requiring analysis. Sorting decisions are stored in a read-only memory semiconductor device. All the input signals for a given event are reexpressed as either 0 or 1 and are used to define an address in this memory which contains the desired sorting outcome for the event. A set of switches enables one to choose between many different procedures for relating input cell pulses to sorting outcomes by switching to different blocks of the memory. The circuitry has been found capable of purity of cell separation to the level permitted by "deadtime" in the preceding analog stages. It is in regular use with the Ontario Cancer Institute flow cytometer-cell sorter.

UI	MeSH Term	Description	Entries
D008863	Microspheres	Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.	Latex Beads,Latex Particles,Latex Spheres,Microbeads,Bead, Latex,Beads, Latex,Latex Bead,Latex Particle,Latex Sphere,Microbead,Microsphere,Particle, Latex,Particles, Latex,Sphere, Latex,Spheres, Latex
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D003201	Computers	Programmable electronic devices designed to accept data, perform prescribed mathematical and logical operations at high speed, and display the results of these operations.	Calculators, Programmable,Computer Hardware,Computers, Digital,Hardware, Computer,Calculator, Programmable,Computer,Computer, Digital,Digital Computer,Digital Computers,Programmable Calculator,Programmable Calculators
D004559	Electric Wiring	An arrangement of wires distributing electricity.	Electrical Wiring,Wiring, Electric,Wiring, Electrical
D004560	Electricity	The physical effects involving the presence of electric charges at rest and in motion.
D005434	Flow Cytometry	Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.	Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell