BIOMAT Database Logo BIOMAT Database Logo

Structure and function of ovotransferrin. II. Iron-transferring activity of iron-binding fragments of ovotransferrin with chicken embryo red cells. 1982

W M Keung, and P Azari

The iron-transferring activity of iron-binding fragments FeNF and FeCF, representing the NH2- and CO2H-terminal domains of iron-ovotransferrin (Fe2OT), with a chicken embryo red cell system, was investigated. Each fragment is able to deliver iron to a chicken embryo red cell system for the biosynthesis of heme, FeNF being 44% more efficient than FeCF. The Fe fragments are on an average approximately 2 times less effective in delivering iron to the chicken embryo red cell than Fe2OT. The binding of FeNF and FeCF to CERC is essentially identical in terms of apparent association constant (K = 1.5 X 10(6) M-1, with respect to fragments) and the number of binding sites per cell at equilibrium (n = 2.9 X 10(4)); however, they differ significantly in their rates of delivering iron for heme synthesis.

UI MeSH Term Description Entries
D007501 Iron A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN. Iron-56,Iron 56
D007700 Kinetics The rate dynamics in chemical or physical systems.
D010446 Peptide Fragments Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques. Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002642 Chick Embryo The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching. Embryo, Chick,Chick Embryos,Embryos, Chick
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D003207 Conalbumin A glycoprotein albumin from hen's egg white with strong iron-binding affinity. Ovotransferrin
D004527 Egg Proteins Proteins which are found in eggs (OVA) from any species. Egg Protein,Egg Shell Protein,Egg Shell Proteins,Egg White Protein,Egg White Proteins,Egg Yolk Protein,Egg Yolk Proteins,Ovum Protein,Ovum Proteins,Yolk Protein,Yolk Proteins,Protein, Egg,Protein, Egg Shell,Protein, Egg White,Protein, Egg Yolk,Protein, Ovum,Protein, Yolk,Proteins, Egg,Proteins, Egg Shell,Proteins, Egg White,Proteins, Egg Yolk,Proteins, Ovum,Proteins, Yolk,Shell Protein, Egg,Shell Proteins, Egg,White Protein, Egg,White Proteins, Egg,Yolk Protein, Egg,Yolk Proteins, Egg
D004912 Erythrocytes Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN. Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

W M Keung, and P Azari
Binding of various ovotransferrin fragments to chick-embryo red cells.
January 1989, The Biochemical journal,
W M Keung, and P Azari
Interaction of oxidized chicken ovotransferrin with chicken embryo red blood cells.
March 1985, Biochimica et biophysica acta,
W M Keung, and P Azari
Binding and iron delivering of ovotransferrin to cholesterol-depleted chick-embryo red blood cells.
January 1995, Cellular signalling,
W M Keung, and P Azari
Iron-binding fragments from the carboxyl-terminal region of hen ovotransferrin.
July 1975, The Biochemical journal,
W M Keung, and P Azari
Polymeric ovotransferrin and its ability to bind and deliver iron to chick-embryo red blood cells.
October 1992, Biochemistry international,
W M Keung, and P Azari
The formation of iron-binding fragments of hen ovotransferrin by limited proteolysis.
September 1974, The Biochemical journal,
W M Keung, and P Azari
On the sturcture of ovotransferrin. 3. Nitration of iron--ovotransferrin and distribution of tyrosines involved in iron-binding activity.
January 1974, Biochemistry,
W M Keung, and P Azari
The iron-binding properties of hen ovotransferrin.
August 1978, The Biochemical journal,
W M Keung, and P Azari
On the structure of ovotransferrin. II. Isolation and characterization of a specific iron-binding fragment after cyanogen bromide cleavage.
January 1974, Biochemistry,
W M Keung, and P Azari
Binding of Cu(II), Tb(III) and Fe(III) to chicken ovotransferrin. A kinetic study.
January 1990, European biophysics journal : EBJ,
Copied contents to your clipboard!