Biomaterial Database

Lateral mobility of phospholipids in turkey erythrocytes. Implications for adenylate cyclase activation. 1982

Y I Henis, and G Rimon, and S Felder

Hormone activation of turkey erythrocyte adenylate cyclase is affected by temperature and by cis-vaccenic acid incorporation. This was correlated with changes in membrane "fluidity" which were previously assumed to affect lateral diffusion of the beta-adrenergic receptor (Rimon, G., Hanski, E., and Levitzki, A. (1980) Biochemistry 19, 4451-4460; Briggs, M. M., and Lefkowitz, R. J. (1980) Biochemistry 19, 4461-4466). To test this hypothesis, we measured the lateral mobility of the fluorescent phospholipid N-4-nitrobenzo-2-oxa-1,3-diazolyl-phosphatidylethanolamine (NBD-PE in turkey erythrocyte ghosts, using fluorescence photobleaching recovery. Ghosts labeled only in the external leaflet exhibited a linear Arrhenius plot of the lateral diffusion coefficient (D) with little change in the mobile fraction of NBD-PE between 45 and 5 degrees C. Labeling from both sides, however, yielded a break point (approximately 30 degrees C) in the Arrhenius plot and a 30% decrease in the mobile fraction of NBD-PE from 24 to 10 degrees C, indicating differences between the external and inner monolayers. The observed discontinuities are not due to gel-phase formation, since fluorescence depolarization of cis- and transparinaric acid did not reveal discontinuities at this temperature range. cis-Vaccenic acid incorporation did not markedly affect either D or the mobile fraction of NBD-PE at 25 degrees C. These results are discussed in view of the effects of temperature and cis-vaccenic acid incorporation on adenylate cyclase activation.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008563	Membrane Lipids	Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.	Cell Membrane Lipid,Cell Membrane Lipids,Membrane Lipid,Lipid, Cell Membrane,Lipid, Membrane,Lipids, Cell Membrane,Lipids, Membrane,Membrane Lipid, Cell,Membrane Lipids, Cell
D010743	Phospholipids	Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.	Phosphatides,Phospholipid
D004789	Enzyme Activation	Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.	Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D004910	Erythrocyte Membrane	The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.	Erythrocyte Ghost,Red Cell Cytoskeleton,Red Cell Ghost,Erythrocyte Cytoskeleton,Cytoskeleton, Erythrocyte,Cytoskeleton, Red Cell,Erythrocyte Cytoskeletons,Erythrocyte Ghosts,Erythrocyte Membranes,Ghost, Erythrocyte,Ghost, Red Cell,Membrane, Erythrocyte,Red Cell Cytoskeletons,Red Cell Ghosts
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D000262	Adenylyl Cyclases	Enzymes of the lyase class that catalyze the formation of CYCLIC AMP and pyrophosphate from ATP.	Adenyl Cyclase,Adenylate Cyclase,3',5'-cyclic AMP Synthetase,Adenylyl Cyclase,3',5' cyclic AMP Synthetase,AMP Synthetase, 3',5'-cyclic,Cyclase, Adenyl,Cyclase, Adenylate,Cyclase, Adenylyl,Cyclases, Adenylyl,Synthetase, 3',5'-cyclic AMP
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures