Biomaterial Database

Variability in the measurement of specific immunoglobulin E antibody by the RAST procedure. 1982

R Evans

This is a report of a study from the Committee on In Vitro Tests of the American Academy of Allergy, which evaluated variance of the RAST procedure when different allergic sera were tested by 12 Committee member laboratories using the Phadebas RAST kit (Pharmacia Diagnostics, Piscataway, N.J.) and when serial dilutions of a ragweed allergic sera were sent to nine different commercial laboratories in the U.S.A. The Phadebas RAST was found to differentiate high-, medium-, and low-titer allergic sera. The test was not useful in measuring smaller changes (less than 61%) in specific IgE antibody that might result from treatment with immunotherapy. Alternative methods of reporting the data, percent total activity and percent negative control, were not superior to the Phadebas RAST unit. Four of the commercial laboratories surveyed did not differentiate by their own measure of significance medium- from low-titer ragweed allergic sera.

UI	MeSH Term	Description	Entries
D006967	Hypersensitivity	Altered reactivity to an antigen, which can result in pathologic reactions upon subsequent exposure to that particular antigen.	Allergy,Allergic Reaction,Allergic Reactions,Allergies,Hypersensitivities,Reaction, Allergic,Reactions, Allergic
D007073	Immunoglobulin E	An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).	IgE
D011852	Radioallergosorbent Test	An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.	Radioimmunosorbent Assay of Allergens,Allergens Radioimmunosorbent Assay,Allergens Radioimmunosorbent Assays,Radioallergosorbent Tests,Test, Radioallergosorbent,Tests, Radioallergosorbent
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000528	Alternaria	A mitosporic Loculoascomycetes fungal genus including several plant pathogens and at least one species which produces a highly phytotoxic antibiotic. Its teleomorph is Lewia.	Alternarias
D019411	Clinical Laboratory Techniques	Techniques used to carry out clinical investigative procedures in the diagnosis and therapy of disease.	Clinical Laboratory Test,Clinical Laboratory Testing,Clinical Laboratory Diagnoses,Clinical Laboratory Testings,Clinical Laboratory Tests,Diagnoses and Laboratory Examinations,Diagnosis, Laboratory,Laboratory Diagnosis,Laboratory Examinations and Diagnoses,Laboratory Techniques, Clinical,Clinical Laboratory Technique,Diagnose, Clinical Laboratory,Laboratory Diagnoses,Laboratory Technique, Clinical,Laboratory Test, Clinical,Laboratory Testing, Clinical,Technique, Clinical Laboratory,Test, Clinical Laboratory,Testing, Clinical Laboratory