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Chemical mutagenesis was studied on Chinese hamster ovary cells by protein mapping. Cell cultures were treated with methylnitrosourea and the cells were cloned in non-selective media. The proteins of single clones were separated by 2-dimensional electrophoresis and analysed for qualitative (electrophoretic mobility) and quantitative (staining intensity; presence/absence) changes in the protein patterns. The investigation included 26 clones derived from treated cells and 26 control clones. The total number of gene loci tested was calculated from the number of protein spots analysed: it amounted to about 33 800. The protein patterns revealed 2 alterations defined as qualitative variant proteins. No alteration of this type was found in the control group. The frequency of quantitative variant proteins was increased by more than 100% compared with the control group. Our results and theoretical considerations suggest that the cellular concentration of single proteins offers a sensitive parameter for mutagenicity testing.
E Zeindl, and
K Sperling, and
J Klose
January 1982,
Mutation research,
E Zeindl, and
K Sperling, and
J Klose
January 1996,
Teratogenesis, carcinogenesis, and mutagenesis,
E Zeindl, and
K Sperling, and
J Klose
January 1979,
Advances in ophthalmology = Fortschritte der Augenheilkunde = Progres en ophtalmologie,
E Zeindl, and
K Sperling, and
J Klose
June 1978,
Proceedings of the National Academy of Sciences of the United States of America,
E Zeindl, and
K Sperling, and
J Klose
March 1983,
Mutation research,
E Zeindl, and
K Sperling, and
J Klose
May 1978,
Mutation research,
E Zeindl, and
K Sperling, and
J Klose
January 1987,
Methods in enzymology,
E Zeindl, and
K Sperling, and
J Klose
February 1985,
Shigaku = Odontology; journal of Nihon Dental College,
E Zeindl, and
K Sperling, and
J Klose
March 1990,
Mutagenesis,
E Zeindl, and
K Sperling, and
J Klose
November 1989,
The Journal of biological chemistry,
