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Applying 2 M KBr, membranes of Ehu were solubilized. By C3-affinity chromatography an activity could be isolated that inhibited the immune adherence reaction and C3b-dependent rosette formation. Since this material did not agglutinate EAC14oxy23b we termed it monovalent C3b receptor (mC3bR). PAGE and SDS-PAGE and staining with Coomassie brilliant blue and PAS reagent revealed a single glycoprotein band with a mol. wt. of 55,000-60,000 daltons and an electrophoretic mobility comparable to ovalbumin. This mC3bR proved to be antigenetically related to gp 205 [17]. The potential of mC3bR to react with C3b-carrying particles was not destroyed by heat and trypsin treatment but by neuraminidase or periodic acid treatment suggesting that mC3bR reacted by its carbohydrate moiety with C3b. As by mC3bR, immune adherence could be inhibited by D-glucose and D-galactose but not by their optical antipodes, L-glucose and L-galactose.
H H Mussel, and
T Ehlen, and
M Schmitt, and
M D Kazatchkine, and
L Neyses, and
M P Dierich
June 1976,
The Japanese journal of experimental medicine,
H H Mussel, and
T Ehlen, and
M Schmitt, and
M D Kazatchkine, and
L Neyses, and
M P Dierich
December 1980,
Immunology,
H H Mussel, and
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M Schmitt, and
M D Kazatchkine, and
L Neyses, and
M P Dierich
June 1982,
Biochemical and biophysical research communications,
H H Mussel, and
T Ehlen, and
M Schmitt, and
M D Kazatchkine, and
L Neyses, and
M P Dierich
September 1979,
Biochimica et biophysica acta,
H H Mussel, and
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M Schmitt, and
M D Kazatchkine, and
L Neyses, and
M P Dierich
October 1981,
Journal of immunology (Baltimore, Md. : 1950),
H H Mussel, and
T Ehlen, and
M Schmitt, and
M D Kazatchkine, and
L Neyses, and
M P Dierich
June 1985,
Journal of immunology (Baltimore, Md. : 1950),
H H Mussel, and
T Ehlen, and
M Schmitt, and
M D Kazatchkine, and
L Neyses, and
M P Dierich
January 1976,
Biochemistry,
H H Mussel, and
T Ehlen, and
M Schmitt, and
M D Kazatchkine, and
L Neyses, and
M P Dierich
December 1981,
FEBS letters,
H H Mussel, and
T Ehlen, and
M Schmitt, and
M D Kazatchkine, and
L Neyses, and
M P Dierich
November 1976,
The Journal of biological chemistry,
H H Mussel, and
T Ehlen, and
M Schmitt, and
M D Kazatchkine, and
L Neyses, and
M P Dierich
August 1983,
Carbohydrate research,
