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A highly sensitive method for the assay of dopamine beta-hydroxylase in rat serum and in sample solution prepared from rat adrenal medulla is described which employs high-performance liquid chromatography with fluorescence detection. Octopamine, formed enzymatically from the substrate tyramine, is separated by Dowex 50/-X4 column chromatography and oxidized with periodate to p-hydroxybenzaldehyde, which is then converted into a fluorescent compound with 2,2'-dithiobis(1-aminonaphthalene). The derivative, after extraction with n-hexane--chloroform, is separated by normal-phase chromatography on Alox T. The limit of detection for octopamine formed enzymatically is 10 pmol. This method requires as little as 5 microliter of rat serum.
H Nohta, and
K Ohtsubo, and
K Zaitsu, and
Y Ohkura
September 1977,
Analytical biochemistry,
H Nohta, and
K Ohtsubo, and
K Zaitsu, and
Y Ohkura
October 1987,
Journal of chromatography,
H Nohta, and
K Ohtsubo, and
K Zaitsu, and
Y Ohkura
April 1987,
Journal of chromatography,
H Nohta, and
K Ohtsubo, and
K Zaitsu, and
Y Ohkura
May 1996,
Biological & pharmaceutical bulletin,
H Nohta, and
K Ohtsubo, and
K Zaitsu, and
Y Ohkura
April 1984,
Biochemical medicine,
H Nohta, and
K Ohtsubo, and
K Zaitsu, and
Y Ohkura
December 1982,
Journal of chromatography,
H Nohta, and
K Ohtsubo, and
K Zaitsu, and
Y Ohkura
May 1985,
Journal of chromatography,
H Nohta, and
K Ohtsubo, and
K Zaitsu, and
Y Ohkura
February 1986,
Journal of pharmacological methods,
H Nohta, and
K Ohtsubo, and
K Zaitsu, and
Y Ohkura
January 1992,
Ophthalmic research,
H Nohta, and
K Ohtsubo, and
K Zaitsu, and
Y Ohkura
November 1987,
Journal of chromatography,
