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Monoclonal antibodies directed toward human PRL (hPRL) have been produced by fusion of mouse myeloma cells (Sp2/0-Ag 14) with spleen cells from mice immunized with hPRL. Total immunizing doses of 20 microgram and 64 microgram hPRL resulted in the production of three highly specific hPRL antibodies. The high affinity antibody, with a Ka value of 0.23 X 10(10) M-1, was used to establish a RIA highly suitable for the measurement of hPRL levels in human serum. The correlation of serum hPRL levels measured using the antibody and those in a conventional rabbit anti-hPRL assay was 0.99 (y = 1.16 - 7.2). These results demonstrate that using the mouse hybridoma technique, it is possible to produce high affinity monospecific monoclonal antibody suitable for the measurement of hPRL in human serum.
M C Stuart, and
P A Underwood, and
L Boscato
February 1983,
Horumon to rinsho. Clinical endocrinology,
M C Stuart, and
P A Underwood, and
L Boscato
January 1977,
La Ricerca in clinica e in laboratorio,
M C Stuart, and
P A Underwood, and
L Boscato
August 1971,
Proceedings of the National Academy of Sciences of the United States of America,
M C Stuart, and
P A Underwood, and
L Boscato
October 1986,
Endocrinologia japonica,
M C Stuart, and
P A Underwood, and
L Boscato
December 1994,
General and comparative endocrinology,
M C Stuart, and
P A Underwood, and
L Boscato
March 1973,
The Journal of clinical endocrinology and metabolism,
M C Stuart, and
P A Underwood, and
L Boscato
September 1989,
Annals of clinical biochemistry,
M C Stuart, and
P A Underwood, and
L Boscato
January 1984,
Journal of immunoassay,
M C Stuart, and
P A Underwood, and
L Boscato
November 1993,
International journal of cancer,
M C Stuart, and
P A Underwood, and
L Boscato
March 1985,
Journal of steroid biochemistry,
