Biomaterial Database

Inorganic phosphate uptake by protoplasts and whole cells of yeast Candida tropicalis: absence of high affinity transport system in protoplasts. 1982

R Jeanjean, and S Bedu, and A Attia, and J Rocca-Serra

Pi-uptake by protoplasts obtained from the yeast Candida tropicalis as well as in whole cells is an energy dependent process. However, the apparent affinity constant of Pi transport by protoplasts about 0.4-0.5 mM whereas Km of 5-6 microM, occurs in whole cells as previously shown. Therefore it can be concluded that protoplasts take up actively Pi but the high affinity capacities are lost. In addition, the Pi-binding proteins are released during protoplasts formation. The presence of Pi-binding proteins in the "cellulase fluid" is demonstrated by immunological tests and by their binding activities after partial purification. These results are in good agreement with the involvement of Pi-binding proteins in Pi uptake in the yeast Candida tropicalis.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D010710	Phosphates	Inorganic salts of phosphoric acid.	Inorganic Phosphate,Phosphates, Inorganic,Inorganic Phosphates,Orthophosphate,Phosphate,Phosphate, Inorganic
D011523	Protoplasts	The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.	Protoplast
D002175	Candida	A genus of yeast-like mitosporic Saccharomycetales fungi characterized by producing yeast cells, mycelia, pseudomycelia, and blastophores. It is commonly part of the normal flora of the skin, mouth, intestinal tract, and vagina, but can cause a variety of infections, including CANDIDIASIS; ONYCHOMYCOSIS; VULVOVAGINAL CANDIDIASIS; and CANDIDIASIS, ORAL (THRUSH).	Candida guilliermondii var. nitratophila,Candida utilis,Cyberlindnera jadinii,Hansenula jadinii,Lindnera jadinii,Monilia,Pichia jadinii,Saccharomyces jadinii,Torula utilis,Torulopsis utilis,Monilias
D002352	Carrier Proteins	Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes.	Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002473	Cell Wall	The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.	Cell Walls,Wall, Cell,Walls, Cell
D002480	Cellulase	An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.	Endo-1,4-beta-Glucanase,Cellulysin,Endoglucanase,Endoglucanase A,Endoglucanase C,Endoglucanase E,Endoglucanase IV,Endoglucanase Y,beta-1,4-Glucan-4-Glucanohydrolase,Endo 1,4 beta Glucanase,beta 1,4 Glucan 4 Glucanohydrolase
D001693	Biological Transport, Active	The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.	Active Transport,Uphill Transport,Active Biological Transport,Biologic Transport, Active,Transport, Active Biological,Active Biologic Transport,Transport, Active,Transport, Active Biologic,Transport, Uphill
D028044	Phosphate-Binding Proteins	Proteins that bind to and are involved in the metabolism of phosphate ions.	Phosphate Binding Protein,Binding Protein, Phosphate,Phosphate Binding Proteins,Protein, Phosphate Binding