-transparent.png){kind=logo}
We describe spectrophotometric determination of methemoglobin (Met-Hb) in blood by absorbance differences at two wavelengths (characteristic of the instrument used) in the 500-600 nm region. Blood is diluted 100- to 200-fold with a solution containing KCN and carbon monoxide to convert hemoglobin to carboxyhemoglobin and Met-Hb to cyanomethemoglobin (CN-Met-Hb). Carboxyhemoglobin has the same absorbance at the two wavelengths used, so that the differences in absorbance reflect only the CN-Met-Hb component. After measurement of absorbance differences, potassium ferricyanide is added to convert all hemoglobin derivatives to CN-Met-Hb; samples are then remeasured at the same wavelengths. The percentage of Met-Hb is determined from the ratio of the absorbance differences. Results agree satisfactorily with currently accepted procedures and with calculated theoretical results for mixtures of oxyhemoglobin, carboxyhemoglobin, and Met-Hb.
M Sakata, and
A Yoshida, and
M Haga
October 1987,
Clinical chemistry,
M Sakata, and
A Yoshida, and
M Haga
January 1999,
Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University,
M Sakata, and
A Yoshida, and
M Haga
May 1975,
Clinical chemistry,
M Sakata, and
A Yoshida, and
M Haga
February 1974,
Clinical chemistry,
M Sakata, and
A Yoshida, and
M Haga
March 1981,
Rinsho byori. The Japanese journal of clinical pathology,
M Sakata, and
A Yoshida, and
M Haga
January 1986,
Journal of pharmaceutical and biomedical analysis,
M Sakata, and
A Yoshida, and
M Haga
May 1970,
American journal of clinical pathology,
M Sakata, and
A Yoshida, and
M Haga
March 2002,
Journal of analytical toxicology,
M Sakata, and
A Yoshida, and
M Haga
December 1956,
American journal of clinical pathology,
M Sakata, and
A Yoshida, and
M Haga
March 1964,
The Journal of clinical endocrinology and metabolism,
