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Antithrombin III (AT-III) was isolated by heparin affinity chromatography from adult venous and newborn term and preterm umbilical cord blood. The purified proteins were compared by SDS-PAGE, rocket immuno-electrophoresis, protein concentration by microbiuret relative to optical density at 280 nm, heparin cofactor specific activity, progressive neutralization of thrombin and factor Xa at 37 degree C and pH related antithrombin kinetics. The structural evaluations revealed a fetal AT-III of molecular weight, charge and electrophoretic migration indistinguishable from adult AT-III. The functional studies showed that, on an equimolar basis, the rates of thrombin and Xa interactions with fetal AT-III were as rapid as those with adult AT-III. The catalytic rates of various concentrations of heparin were also equal. The newborn infant, therefore, displays a quantitative but not quantitative deficiency of AT-III.
M M McDonald, and
W E Hathaway, and
E B Reeve, and
B D Leonard
January 1994,
Acta obstetricia et gynecologica Scandinavica,
M M McDonald, and
W E Hathaway, and
E B Reeve, and
B D Leonard
February 1984,
European journal of pediatrics,
M M McDonald, and
W E Hathaway, and
E B Reeve, and
B D Leonard
June 1982,
Boletin medico del Hospital Infantil de Mexico,
M M McDonald, and
W E Hathaway, and
E B Reeve, and
B D Leonard
January 1984,
Haematologia,
M M McDonald, and
W E Hathaway, and
E B Reeve, and
B D Leonard
July 1991,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
M M McDonald, and
W E Hathaway, and
E B Reeve, and
B D Leonard
August 1990,
Biochimie,
M M McDonald, and
W E Hathaway, and
E B Reeve, and
B D Leonard
February 1985,
Pediatric research,
M M McDonald, and
W E Hathaway, and
E B Reeve, and
B D Leonard
June 1987,
Thrombosis and haemostasis,
M M McDonald, and
W E Hathaway, and
E B Reeve, and
B D Leonard
February 1980,
Scandinavian journal of haematology,
M M McDonald, and
W E Hathaway, and
E B Reeve, and
B D Leonard
July 1987,
Thrombosis research,
