Biomaterial Database

Characterization of a human cryoglobulin complex: a crystalline adduct of a monoclonal immunoglobulin G and albumin. 1982

J E Jentoft, and D G Dearborn, and G M Bernier

An unusual human cryoglobulin complex was characterized as a two-component system containing monoclonal immunoglobulin G (IgG) and serum albumin in a 1:2 mole ratio. This complex appeared to be an antibody-antigen complex, since the mole ratio was appropriate and the isolated Fab of the IgG associated with the albumin. The cryoglobulin apparently arose as a result of specific association and/or aggregation of the IgG albumin adduct, since the cryoglobulin complex formed a crystalline precipitate. The IgG and albumin were separated and characterized with respect to immunological cross-reactivities, sedimentation velocities, isoelectric properties, and amino acid composition. The extent of precipitation of the cryoglobulin complex was maximal at pH 7.8, was decreased by added ions including citrate, ethylenediaminetetraacetic acid, NaCl, and CaCl2, and was decreased by increasing temperature. Both the nature of the cold-precipitable complex and the solubility properties differed from those described for other cryoglobulins.

UI	MeSH Term	Description	Entries
D007074	Immunoglobulin G	The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.	Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D003450	Cryoglobulins	Abnormal immunoglobulins, especially IGG or IGM, that precipitate spontaneously when SERUM is cooled below 37 degrees Celsius. It is characteristic of CRYOGLOBULINEMIA.	Cryoglobulin,Cryoprotein,Cryoproteins
D003461	Crystallography	The branch of science that deals with the geometric description of crystals and their internal arrangement. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Crystallographies
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000936	Antigen-Antibody Complex	The complex formed by the binding of antigen and antibody molecules. The deposition of large antigen-antibody complexes leading to tissue damage causes IMMUNE COMPLEX DISEASES.	Immune Complex,Antigen-Antibody Complexes,Immune Complexes,Antigen Antibody Complex,Antigen Antibody Complexes,Complex, Antigen-Antibody,Complex, Immune,Complexes, Antigen-Antibody,Complexes, Immune
D012709	Serum Albumin	A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.	Plasma Albumin,Albumin, Serum
D012995	Solubility	The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Solubilities