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We have prepared a dry film for the enzymic determination of total serum cholesterol. It consists of a transparent support bearing a buffered gelatin layer, and a white reflective spreading layer that contains all of the necessary components for the detection of cholesterol. The method is based on (a) hydrolysis of cholesterol esters to cholesterol by cholesterol ester hydrolase (EC 3.1.1.13), (b) oxidation of cholesterol to cholest-4-en-4-one and hydrogen peroxide by cholesterol oxidase (EC 1.1.3.6), and (c) oxidation of a triarylimidazole leuco dye with hydrogen peroxide in the presence of peroxidase (EC 1.11.1.7) to produce a dye with maximum absorption at about 650 nm. For use over a wider range of concentration, the dye density is read at 540 nm. With reflection densitometry and appropriate mathematical transformation, readings and cholesterol concentrations are linearly related to 5500 mg/L. Results correlate well with those by the Abell-Kendall comparison method (slope 0.97, intercept 92.5, correlation coefficient 0.974, Sy.x = 250.7), and the method is precise (CV of 1.2-2.3% for a control fluid and patients' samples) and relatively free of interferences.
G M Dappen, and
P E Cumbo, and
C T Goodhue, and
S Y Lynn, and
C C Morganson, and
B F Nellis, and
D M Sablauskas, and
J R Schaeffer, and
R M Schubert, and
R E Snoke, and
G M Underwood, and
C D Warburton, and
T W Wu
October 1983,
Clinical chemistry,
G M Dappen, and
P E Cumbo, and
C T Goodhue, and
S Y Lynn, and
C C Morganson, and
B F Nellis, and
D M Sablauskas, and
J R Schaeffer, and
R M Schubert, and
R E Snoke, and
G M Underwood, and
C D Warburton, and
T W Wu
January 1983,
Clinical chemistry,
G M Dappen, and
P E Cumbo, and
C T Goodhue, and
S Y Lynn, and
C C Morganson, and
B F Nellis, and
D M Sablauskas, and
J R Schaeffer, and
R M Schubert, and
R E Snoke, and
G M Underwood, and
C D Warburton, and
T W Wu
November 1975,
Zeitschrift fur klinische Chemie und klinische Biochemie,
G M Dappen, and
P E Cumbo, and
C T Goodhue, and
S Y Lynn, and
C C Morganson, and
B F Nellis, and
D M Sablauskas, and
J R Schaeffer, and
R M Schubert, and
R E Snoke, and
G M Underwood, and
C D Warburton, and
T W Wu
November 1983,
Clinical chemistry,
G M Dappen, and
P E Cumbo, and
C T Goodhue, and
S Y Lynn, and
C C Morganson, and
B F Nellis, and
D M Sablauskas, and
J R Schaeffer, and
R M Schubert, and
R E Snoke, and
G M Underwood, and
C D Warburton, and
T W Wu
January 1978,
Clinical chemistry,
G M Dappen, and
P E Cumbo, and
C T Goodhue, and
S Y Lynn, and
C C Morganson, and
B F Nellis, and
D M Sablauskas, and
J R Schaeffer, and
R M Schubert, and
R E Snoke, and
G M Underwood, and
C D Warburton, and
T W Wu
November 1985,
Clinical chemistry,
G M Dappen, and
P E Cumbo, and
C T Goodhue, and
S Y Lynn, and
C C Morganson, and
B F Nellis, and
D M Sablauskas, and
J R Schaeffer, and
R M Schubert, and
R E Snoke, and
G M Underwood, and
C D Warburton, and
T W Wu
May 1974,
Zeitschrift fur klinische Chemie und klinische Biochemie,
G M Dappen, and
P E Cumbo, and
C T Goodhue, and
S Y Lynn, and
C C Morganson, and
B F Nellis, and
D M Sablauskas, and
J R Schaeffer, and
R M Schubert, and
R E Snoke, and
G M Underwood, and
C D Warburton, and
T W Wu
July 1964,
Das Medizinische Laboratorium,
G M Dappen, and
P E Cumbo, and
C T Goodhue, and
S Y Lynn, and
C C Morganson, and
B F Nellis, and
D M Sablauskas, and
J R Schaeffer, and
R M Schubert, and
R E Snoke, and
G M Underwood, and
C D Warburton, and
T W Wu
November 1964,
"Laboratorio;" analisis clinicos, bacteriologia, inmunologia, parasitologia, hematologia, anatomia patologica, quimica clinica,
G M Dappen, and
P E Cumbo, and
C T Goodhue, and
S Y Lynn, and
C C Morganson, and
B F Nellis, and
D M Sablauskas, and
J R Schaeffer, and
R M Schubert, and
R E Snoke, and
G M Underwood, and
C D Warburton, and
T W Wu
July 1978,
Clinical chemistry,
