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Peripheral blood mononuclear cells (PBMCs) from three patients (a mother and her two children) with acute natural rubella were obtained five to nine days after the onset of rash. After mitogenic stimulation for three days in culture, the PBMCs were cocultivated with rabbit kidney cells (cell line RK13) at 35 C. Widespread cytopathologic effects on the monolayer were apparent by day 7 after cocultivation, and analysis of the supernatant revealed titers of 10(7) plaque-forming units of rubella virus (RV)/ml. Viral particles were also detected by electron microscopy, and the major RV polypeptides were shown to be present by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. One month later an identical study showed that neither RV polypeptides nor infectious virus was present in the PBMCs, a result which indicated that lymphocyte viremia had been controlled.
J K Chantler, and
A J Tingle
July 1979,
Infection and immunity,
J K Chantler, and
A J Tingle
April 1983,
Infection and immunity,
J K Chantler, and
A J Tingle
January 1975,
Lancet (London, England),
J K Chantler, and
A J Tingle
November 1968,
Lancet (London, England),
J K Chantler, and
A J Tingle
March 1992,
The Pediatric infectious disease journal,
J K Chantler, and
A J Tingle
March 1946,
The Journal of clinical investigation,
J K Chantler, and
A J Tingle
April 1964,
Nederlands tijdschrift voor geneeskunde,
J K Chantler, and
A J Tingle
May 1970,
British medical journal,
J K Chantler, and
A J Tingle
April 1975,
The Journal of infectious diseases,
J K Chantler, and
A J Tingle
November 1977,
British medical journal,
