Biomaterial Database

Effects of the sites of injection of aminoguanidine sulfate on developing eggs. 1982

T Sugiyama, and N Suguro, and A Hayashida

The difference in biological effects and distribution when aminoguanidine sulfate (AGS) was injected into different sites of developing eggs was studied. (I) Five mg to 20 mg of AGS were injected once into the air sac (AS), albumen (AL) or yolk sac (YS) from the 4th to the 7th day of incubation. Observation continued up to the 18th day of incubation. In the AS group, total mortality was the highest and external abnormalities were most frequent; however, abnormal findings in liver were infrequent and slight. In the YS and particularly in the AL group, on the other hand, liver abnormalities such as low weight, degeneration, necrosis, increase of mitosis, delay of development and fibrosis were found. These are considered as specific effects of AGS. These frequencies were high when AGS was injected on the 4th and 5th day of incubation. (II) Twenty mg injection of AGS into the AS, AL, or YS on the 5th or 8th day of incubation was made for a distribution study. AGS concentration was determined in the various compartments as various times after injection. Distribution of AGS in the AS group was significantly different from that in the AL and YS groups. The AL and YS groups, however, differed a little. The exact route which AGS reaches from the site of injection to the chick embryos could not be determined, but it is concluded that the albumen is the best site for the injection of AGS to investigate its specific effects on liver.

UI	MeSH Term	Description	Entries
D007267	Injections	Introduction of substances into the body using a needle and syringe.	Injectables,Injectable,Injection
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D010047	Ovalbumin	An albumin obtained from the white of eggs. It is a member of the serpin superfamily.	Serpin B14
D002642	Chick Embryo	The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.	Embryo, Chick,Chick Embryos,Embryos, Chick
D006146	Guanidines	A family of iminourea derivatives. The parent compound has been isolated from mushrooms, corn germ, rice hulls, mussels, earthworms, and turnip juice. Derivatives may have antiviral and antifungal properties.
D000014	Abnormalities, Drug-Induced	Congenital abnormalities caused by medicinal substances or drugs of abuse given to or taken by the mother, or to which she is inadvertently exposed during the manufacture of such substances. The concept excludes abnormalities resulting from exposure to non-medicinal chemicals in the environment.	Drug-Induced Abnormalities,Abnormalities, Drug Induced,Abnormality, Drug-Induced,Drug Induced Abnormalities,Drug-Induced Abnormality
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D015017	Yolk Sac	The first of four extra-embryonic membranes to form during EMBRYOGENESIS. In REPTILES and BIRDS, it arises from endoderm and mesoderm to incorporate the EGG YOLK into the DIGESTIVE TRACT for nourishing the embryo. In placental MAMMALS, its nutritional function is vestigial; however, it is the source of INTESTINAL MUCOSA; BLOOD CELLS; and GERM CELLS. It is sometimes called the vitelline sac, which should not be confused with the VITELLINE MEMBRANE of the egg.	Vitelline Sac of Embryo,Embryo Vitelline Sac,Embryo Vitelline Sacs,Sac, Yolk,Sacs, Yolk,Yolk Sacs