Biomaterial Database

Polarized amino acid transport by an epithelial cell line of renal origin (LLC-PK1). The basolateral systems. 1982

C A Rabito, and M V Karish

The transport of three neutral amino acid analogs has been studied in an epithelial cell line from a pig kidney. 2-Aminoisobutyric acid is accumulated by LLC-PK1 cells against a concentration gradient through a mechanism with several features of a carrier-mediated process. The influx is accounted for by a saturable Na+-dependent and nonsaturable Na+-independent process. The total influx of 2-(methylamino)isobutyric acid is also mediated through Na+-dependent and Na+-independent systems. Part of the Na+-dependent influx of 2-aminoisobutyric acid was competitively inhibited by 2-(methylamino)isobutyric acid (Ki = 4.2 mM).l Selectivity studies indicate that the 2-(methylamino)isobutyric acid-sensitive part of 2-aminoisobutyric acid influx is mediated through the A system, whereas the insensitive part occurs through the ASC system. Cycloleucine transport also involves a Na+-dependent and Na+-independent process. The Na+-dependent influx is completely inhibited by 2-aminoisobutyric acid but not perceptibly by 2-(methylamino)isobutyric acid. This influx probably represents entry through the ASC system. The Na+-independent component is completely inhibited by 2-aminobicyclo(2,2,1)heptane-2-carboxylic acid, a specific substrate for the L system. Uptake studies from either side of the monolayers indicate that these transport systems are exclusively located in basolateral membranes of the cultured renal cells.

UI	MeSH Term	Description	Entries
D007668	Kidney	Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.	Kidneys
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D010042	Ouabain	A cardioactive glycoside consisting of rhamnose and ouabagenin, obtained from the seeds of Strophanthus gratus and other plants of the Apocynaceae; used like DIGITALIS. It is commonly used in cell biological studies as an inhibitor of the NA(+)-K(+)-EXCHANGING ATPASE.	Acocantherin,G-Strophanthin,Acolongifloroside K,G Strophanthin
D011188	Potassium	An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
D011392	Proline	A non-essential amino acid that is synthesized from GLUTAMIC ACID. It is an essential component of COLLAGEN and is important for proper functioning of joints and tendons.	L-Proline,L Proline
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D004848	Epithelium	The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body.	Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D000596	Amino Acids	Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.	Amino Acid,Acid, Amino,Acids, Amino
D000621	Aminoisobutyric Acids	A group of compounds that are derivatives of the amino acid 2-amino-2-methylpropanoic acid.	Acids, Aminoisobutyric
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia