BIOMAT Database Logo BIOMAT Database Logo

Inhibition of calcium absorption in the cortical thick ascending limb of Henle's loop by furosemide. 1982

J E Bourdeau, and S L Buss, and G G Vurek

The effect of luminal furosemide on calcium transport across the cortical thick ascending limb of Henle's loop was studied. Cortical thick ascending limbs were dissected from rabbit kidneys and perfused in vitro. Calcium concentrations in perfused and collected tubule fluids were measured by continuous-flow microcolorimetry. Tubules were perfused at slow flow rates and parathyroid hormone was added to the bathing solution to maximize the difference between perfusate and collectate calcium concentrations. Furosemide simultaneously and reversibly inhibited the lumen-positive transepithelial voltage and calcium absorption. In the presence of furosemide perfusate-minus-collectate calcium concentration difference was smallest under conditions that minimized the residual transepithelial voltage. It was concluded furosemide inhibits calcium absorption in the cortical thick ascending limb of Henle's loop by reducing the lumen-positive transepithelial voltage that drives passive calcium ion transport.

UI MeSH Term Description Entries
D007672 Kidney Cortex The outer zone of the KIDNEY, beneath the capsule, consisting of KIDNEY GLOMERULUS; KIDNEY TUBULES, DISTAL; and KIDNEY TUBULES, PROXIMAL. Cortex, Kidney
D007684 Kidney Tubules Long convoluted tubules in the nephrons. They collect filtrate from blood passing through the KIDNEY GLOMERULUS and process this filtrate into URINE. Each renal tubule consists of a BOWMAN CAPSULE; PROXIMAL KIDNEY TUBULE; LOOP OF HENLE; DISTAL KIDNEY TUBULE; and KIDNEY COLLECTING DUCT leading to the central cavity of the kidney (KIDNEY PELVIS) that connects to the URETER. Kidney Tubule,Tubule, Kidney,Tubules, Kidney
D008138 Loop of Henle The U-shaped portion of the renal tubule in the KIDNEY MEDULLA, consisting of a descending limb and an ascending limb. It is situated between the PROXIMAL KIDNEY TUBULE and the DISTAL KIDNEY TUBULE. Ascending Limb of Loop of Henle,Descending Limb of Loop of Henle,Henle Loop
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D004558 Electric Stimulation Use of electric potential or currents to elicit biological responses. Stimulation, Electric,Electrical Stimulation,Electric Stimulations,Electrical Stimulations,Stimulation, Electrical,Stimulations, Electric,Stimulations, Electrical
D005260 Female Females
D005665 Furosemide A benzoic-sulfonamide-furan. It is a diuretic with fast onset and short duration that is used for EDEMA and chronic RENAL INSUFFICIENCY. Frusemide,Fursemide,Errolon,Frusemid,Furanthril,Furantral,Furosemide Monohydrochloride,Furosemide Monosodium Salt,Fusid,Lasix
D000042 Absorption The physical or physiological processes by which substances, tissue, cells, etc. take up or take in other substances or energy.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

J E Bourdeau, and S L Buss, and G G Vurek
Bicarbonate absorption by the thick ascending limb of Henle's loop.
March 1990, Seminars in nephrology,
J E Bourdeau, and S L Buss, and G G Vurek
Ammonia absorption by the thick ascending limb of Henle's loop.
January 1985, Contributions to nephrology,
J E Bourdeau, and S L Buss, and G G Vurek
Thick ascending limb of Henle's loop.
November 1982, Kidney international,
J E Bourdeau, and S L Buss, and G G Vurek
Effect of PTH on calcium transport across the cortical thick ascending limb of Henle's loop.
August 1980, The American journal of physiology,
J E Bourdeau, and S L Buss, and G G Vurek
Furosemide directly stimulates prostaglandin E2 production in the thick ascending limb of Henle's loop.
December 1989, The Journal of pharmacology and experimental therapeutics,
J E Bourdeau, and S L Buss, and G G Vurek
Ammonium transport by the thick ascending limb of Henle's loop.
January 1994, Annual review of physiology,
J E Bourdeau, and S L Buss, and G G Vurek
Function of the thick ascending limb of Henle's loop.
March 1973, The American journal of physiology,
J E Bourdeau, and S L Buss, and G G Vurek
Voltage dependence of calcium transport in the thick ascending limb of Henle's loop.
April 1979, The American journal of physiology,
J E Bourdeau, and S L Buss, and G G Vurek
A mathematical model of rabbit cortical thick ascending limb of the Henle's loop.
April 1991, Biochimica et biophysica acta,
J E Bourdeau, and S L Buss, and G G Vurek
Magnesium transport in the cortical thick ascending limb of Henle's loop of the rabbit.
April 1982, The Journal of clinical investigation,
Copied contents to your clipboard!