Simulation and analysis of hepatic indicator dilution curves. 1982

B A Luxon, and E L Forker

The "multiple-indicator dilution method" of measuring hepatic transport kinetics is subjected to simulation analysis. The objective is to examine the errors that may arise from treating the nonexchanging vasculature as a simple delay and to study the information content of simulated venous outflow curves. We find that the method cannot be counted on to provide consistently reliable estimates of either the transport rate constants or the sinusoidal volume. Estimates of the rate constant for irreversible solute removal from within liver cells are especially likely to be wrong. We suggest an alternative formulation of the governing differential equations that can substantially improve the estimates of the uptake parameter. These estimates may otherwise be subject to large systematic errors. Finally we discuss why the steady-state extraction fraction computed from the fitted parameters should be checked against a model-independent estimate obtained directly from the areas under the outflow curves. A method for making this comparison is at hand and should prove useful as a minimum criterion of internal consistency in animal experiments.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008433 Mathematics The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Mathematic
D008722 Methods A series of steps taken in order to conduct research. Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001692 Biological Transport The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments. Transport, Biological,Biologic Transport,Transport, Biologic

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