Biomaterial Database

Use of gonococcal agar medium for preparation of antigen of Haemophilus paragallinarum hemagglutinin. 1982

T Sueishi, and Y Hayashi, and Y Iritani

Some solid media for antigen preparation of Haemophilus paragallinarum (HPG) hemagglutinin (HA) were studied. When HA activities of HPG strain 221 cultivated on chicken meat infusion (CMI) and gonococcal (GC) agar media were compared, the titer of the GC agar medium appeared faster and was higher than the titer on the CMI agar medium. For both agar media, the HA titer and growth increased with the concentration of the chicken serum added. In addition, HA activity on GC agar medium showed a higher titer than activity on other media, such as brain-heart infusion, Trypticase soy, blood, and Kato's agar media. When various HPG strains were inoculated onto GC agar medium, HA titers in strains 0083 and F were equal to that in strain 221. These results confirm that the GC agar medium is useful for preparation of HA antigen of HPG.

UI	MeSH Term	Description	Entries
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D006190	Haemophilus	A genus of PASTEURELLACEAE that consists of several species occurring in animals and humans. Its organisms are described as gram-negative, facultatively anaerobic, coccobacillus or rod-shaped, and nonmotile.	Hemophilus
D006386	Hemagglutination Tests	Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)	Hemagglutination Test,Test, Hemagglutination,Tests, Hemagglutination
D006388	Hemagglutinins	Agents that cause agglutination of red blood cells. They include antibodies, blood group antigens, lectins, autoimmune factors, bacterial, viral, or parasitic blood agglutinins, etc.	Isohemagglutinins,Exohemagglutinins,Hemagglutinin
D000362	Agar	A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000942	Antigens, Bacterial	Substances elaborated by bacteria that have antigenic activity.	Bacterial Antigen,Bacterial Antigens,Antigen, Bacterial