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1. Pig lactate dehydrogenase isoenzyme M4 was labelled with O-(4-diazo-3,5-di[125I]iodobenzoyl)sucrose and injected intravenously into rats. Previous work has shown that this label does not influence the clearance of the enzyme (half-life about 26 min) and that it is retained within the lysosomes for several hours after endocytosis and breakdown of the protein [De Jong, Bouma & Gruber (1981) Biochem. J. 198, 45--51]. 2. The distribution of the radioactivity over a large number of tissues was determined 2 h after injection. A high percentage of the injected dose was found in liver (41%), spleen (10%) and bone including marrow (21%). 3. Autoradiography indicated uptake of the enzyme mainly by Kupffer cells of the liver, by spleen macrophages and by bone marrow macrophages. 4. Liver cells were isolated 1 h after injection of the enzyme. Kupffer cells, endothelial cells and parenchymal cells were found to endocytose the enzyme at rates corresponding to 4230, 35 and 25 ml of plasma/day per g of cell protein, respectively. 5. Previous injection of carbon particles greatly reduced the uptake of the enzyme by liver and spleen, but the uptake by bone marrow was not significantly changed.
A S De Jong, and
A M Duursma, and
J M Bouma, and
M Gruber, and
A Brouwer, and
D L Knook
April 1979,
The Biochemical journal,
A S De Jong, and
A M Duursma, and
J M Bouma, and
M Gruber, and
A Brouwer, and
D L Knook
July 1981,
The Biochemical journal,
A S De Jong, and
A M Duursma, and
J M Bouma, and
M Gruber, and
A Brouwer, and
D L Knook
March 1985,
The Biochemical journal,
A S De Jong, and
A M Duursma, and
J M Bouma, and
M Gruber, and
A Brouwer, and
D L Knook
January 1985,
FEBS letters,
A S De Jong, and
A M Duursma, and
J M Bouma, and
M Gruber, and
A Brouwer, and
D L Knook
November 1980,
The Biochemical journal,
A S De Jong, and
A M Duursma, and
J M Bouma, and
M Gruber, and
A Brouwer, and
D L Knook
September 1981,
The Biochemical journal,
A S De Jong, and
A M Duursma, and
J M Bouma, and
M Gruber, and
A Brouwer, and
D L Knook
September 1998,
Arhiv za higijenu rada i toksikologiju,
A S De Jong, and
A M Duursma, and
J M Bouma, and
M Gruber, and
A Brouwer, and
D L Knook
January 1976,
Metabolism: clinical and experimental,
A S De Jong, and
A M Duursma, and
J M Bouma, and
M Gruber, and
A Brouwer, and
D L Knook
January 1976,
Biokhimiia (Moscow, Russia),
A S De Jong, and
A M Duursma, and
J M Bouma, and
M Gruber, and
A Brouwer, and
D L Knook
January 1967,
Biochimica et biophysica acta,
