-transparent.png){kind=logo}
A method is described for the estimation of hypoxanthine-guanine phosphoribosyltransferase using high performance liquid chromatography. The inosine monophosphate (IMP) generated from hypoxanthine is determined, after separation on a C18 reversed phase silica column with a buffer-methanol gradient, by the absorbance at 254 nm. Simultaneous reciprocal measurement of hypoxanthine consumption is made. The assay is suitable for screening red cell lysates for hypoxanthine-guanine phosphoribosyltransferase deficiency; the results being expressed as nmol inosine monophosphate . h-1. mg-1 haemoglobin. The normal range found was 94 +/- 15 nmol IMP . h-1 . mg-1 haemoglobin and hypoxanthine-guanine phosphoribosyltransferase activities down to 1% of normal can be assayed accurately.
H J Rylance, and
R C Wallace, and
G Nuki
December 1996,
Nihon rinsho. Japanese journal of clinical medicine,
H J Rylance, and
R C Wallace, and
G Nuki
January 1991,
Journal of reproduction and fertility,
H J Rylance, and
R C Wallace, and
G Nuki
February 1976,
Human genetics,
H J Rylance, and
R C Wallace, and
G Nuki
December 1985,
Journal of chromatography,
H J Rylance, and
R C Wallace, and
G Nuki
April 1979,
Biochemical pharmacology,
H J Rylance, and
R C Wallace, and
G Nuki
January 1973,
Advances in experimental medicine and biology,
H J Rylance, and
R C Wallace, and
G Nuki
April 1988,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
H J Rylance, and
R C Wallace, and
G Nuki
February 2007,
Molecular genetics and metabolism,
H J Rylance, and
R C Wallace, and
G Nuki
January 1984,
Advances in experimental medicine and biology,
H J Rylance, and
R C Wallace, and
G Nuki
January 1984,
Advances in experimental medicine and biology,
