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Discontinuous counterimmunoelectrophoresis (DCIE) was employed to detect the toxin of Clostridium difficile, etiologic antibiotic-associated colitis (AAC), in bacteria-free stool filtrates from 51 patients with diarrhea. Stool samples from 31 patients contained C. difficile toxin as determined by tissue-culture assay. A positive result was obtained by DCIE in 20 of the 31 patients (65%) and was influenced by the titer of toxin present. When toxin was present by tissue-culture assay in a dilution of less than or equal to 10(-2) (11 samples), DCIE was positive in only 2 (18%). However, DCIE yielded positive results in 18 of the 20 samples (90%) containing toxin titers greater than or equal to 10(-3). The combination of DCIE and sigmoidoscopy of colonoscopy was superior to either alone in the diagnosis of AAC irrespective of the toxin titer. Nine of 11 patients (82%) whose stool samples contained C. difficile toxin in a dilution of less than or equal to 10(-2) were recognized by DCIE, endoscopy, or both. In stool samples containing toxin in titers greater than or equal to 10(-3), no false-negative results were encountered (sensitivity equals 100%). Thus, 29 of 31 patients whose stool samples contained C. difficile toxin were identified when the results of DCIE and endoscopical examination were combined (sensitivity 93.5%). Neither endoscopical examination nor DCIE yielded positive results in the 20 patients whose stool samples lacked C. difficile toxin (specificity equals 100%). DCIE is a rapid, moderately sensitive, and specific method for detecting C. difficile toxin. When DCIE is combined with endoscopy, the vast majority of patients requiring specific therapy for AAC can be identified.
J F Fisher, and
F J Tedesco, and
D H Johnson, and
J P Rissing, and
C A Walker, and
R C Trincher, and
L Howard, and
T Buxton, and
J F Agel
April 1983,
Journal of clinical microbiology,
J F Fisher, and
F J Tedesco, and
D H Johnson, and
J P Rissing, and
C A Walker, and
R C Trincher, and
L Howard, and
T Buxton, and
J F Agel
January 1981,
Scandinavian journal of gastroenterology,
J F Fisher, and
F J Tedesco, and
D H Johnson, and
J P Rissing, and
C A Walker, and
R C Trincher, and
L Howard, and
T Buxton, and
J F Agel
January 1982,
Schweizerische Rundschau fur Medizin Praxis = Revue suisse de medecine Praxis,
J F Fisher, and
F J Tedesco, and
D H Johnson, and
J P Rissing, and
C A Walker, and
R C Trincher, and
L Howard, and
T Buxton, and
J F Agel
April 1978,
British medical journal,
J F Fisher, and
F J Tedesco, and
D H Johnson, and
J P Rissing, and
C A Walker, and
R C Trincher, and
L Howard, and
T Buxton, and
J F Agel
January 1983,
Scandinavian journal of gastroenterology. Supplement,
J F Fisher, and
F J Tedesco, and
D H Johnson, and
J P Rissing, and
C A Walker, and
R C Trincher, and
L Howard, and
T Buxton, and
J F Agel
January 1980,
The Medical journal of Australia,
J F Fisher, and
F J Tedesco, and
D H Johnson, and
J P Rissing, and
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R C Trincher, and
L Howard, and
T Buxton, and
J F Agel
December 1985,
Comprehensive therapy,
J F Fisher, and
F J Tedesco, and
D H Johnson, and
J P Rissing, and
C A Walker, and
R C Trincher, and
L Howard, and
T Buxton, and
J F Agel
September 1975,
Comprehensive therapy,
J F Fisher, and
F J Tedesco, and
D H Johnson, and
J P Rissing, and
C A Walker, and
R C Trincher, and
L Howard, and
T Buxton, and
J F Agel
July 1981,
Southern medical journal,
J F Fisher, and
F J Tedesco, and
D H Johnson, and
J P Rissing, and
C A Walker, and
R C Trincher, and
L Howard, and
T Buxton, and
J F Agel
January 1983,
Tropical gastroenterology : official journal of the Digestive Diseases Foundation,
