Biomaterial Database

A water-soluble cholesterol derivative for use in augmenting serum control materials. 1978

G J Proksch, and D P Bonderman

Cholesterol determination has been hampered by the lack of suitable means to prepare lyophilized serum control materials with an appropriate range of values. We prepared a water-soluble cholesterol derivative by first esterifying cholesterol with adipic acid and then reacting the cholesterol hemiadipate with Polyethylene Glycol 600 to form polyethoxyethanyl-cholesteryl adipate. The compound reacts quantitatively in several commonly used methods, including enzymic, extraction, and direct-assay procedures. When the additive is directly mixed with human serum that has been depleted of beta- and pre-beta-lipoproteins, an optically clear solution results for which cholesterol values are stable. The clarity is retained upon lyophilization and reconstitution. Addition of this cholesterol compound to partially delipidized serum appeared to have no significant effect on results of assay of 18 other commonly measured serum constituents.

UI	MeSH Term	Description	Entries
D012015	Reference Standards	A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.	Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D002784	Cholesterol	The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.	Epicholesterol
D002788	Cholesterol Esters	Fatty acid esters of cholesterol which constitute about two-thirds of the cholesterol in the plasma. The accumulation of cholesterol esters in the arterial intima is a characteristic feature of atherosclerosis.	Cholesterol Ester,Cholesteryl Ester,Cholesteryl Esters,Ester, Cholesterol,Ester, Cholesteryl,Esters, Cholesterol,Esters, Cholesteryl
D005612	Freeze Drying	Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.	Lyophilization,Drying, Freeze,Dryings, Freeze,Freeze Dryings,Lyophilizations
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012995	Solubility	The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Solubilities