Biomaterial Database

Interference with the kinetic determination of acid phosphatase. 1978

G T Sanders, and P Serné, and F J Hoek

This study advocates cautious use of the kinetic determination method for acid phosphatase with alpha-naphthylphosphate as substrate and a diazonium salt for colour development. The occurrence of considerable blank rates due to the spontaneous hydrolysis of the substrate was noticed. Of the diazonium salts which were tested as alternatives for Fast Red TR, diazotised p-nitroaniline seemed to be useful. Also the interaction between plasma components and diazonium salts may influence the results of the determination. In this respect a number of possible interfering compounds was investigated and from these a structural element which may lead to side reactions was deduced.

UI	MeSH Term	Description	Entries
D007202	Indicators and Reagents	Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)	Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D009281	Naphthalenes	Two-ring crystalline hydrocarbons isolated from coal tar. They are used as intermediates in chemical synthesis, as insect repellents, fungicides, lubricants, preservatives, and, formerly, as topical antiseptics.
D003979	Diazonium Compounds	Azo compounds consisting of an aryl or alkyl group that is joined through two nitrogen atoms to an anion (R-N2+X-).	Compounds, Diazonium
D000135	Acid Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.2.	Acid beta-Glycerophosphatase,Acid beta Glycerophosphatase
D013053	Spectrophotometry	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.