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A new colorimetric determination for serum phospholipid is described. Firstly, serum phospholipid is incubated with phospholipase C from Bacillus cereus, and then the released diglyceride and triglyceride are hydrolyzed completely to fatty acid and glycerol by lipoprotein lipase from Pseudomonas fluorescens. Secondly, the glycerol produced is enzymatically determined by glycerol dehydrogenase in the presence of NAD+, using phenazine methosulfate-nitro blue tetrazolium as color reagents. The absorbance at 570 nm is recorded. The amount of the glycerol from phospholipid is calculated by subtracting the amount of glycerol from triglyceride from the amount of total glycerol. The present method requires only 20 microliter of serum and a 40 min incubation and is highly reproducible. The results obtained show good correlation with those obtained by a chemical method (correlation coefficient, 0.925) or the phospholipase D-choline oxidase method (correlation coefficient, 0.936). These results strongly suggest that the proposed method can be utilized as a routine clinical test.
M Sugiura, and
T Oikawa, and
K Hirano
January 1977,
Clinical chemistry,
M Sugiura, and
T Oikawa, and
K Hirano
December 1987,
Clinical chemistry,
M Sugiura, and
T Oikawa, and
K Hirano
May 1981,
Chemical & pharmaceutical bulletin,
M Sugiura, and
T Oikawa, and
K Hirano
August 1980,
Clinica chimica acta; international journal of clinical chemistry,
M Sugiura, and
T Oikawa, and
K Hirano
February 1979,
Clinical chemistry,
M Sugiura, and
T Oikawa, and
K Hirano
October 1982,
Analytical chemistry,
M Sugiura, and
T Oikawa, and
K Hirano
May 1981,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
M Sugiura, and
T Oikawa, and
K Hirano
January 1981,
Clinical chemistry,
M Sugiura, and
T Oikawa, and
K Hirano
November 1969,
Analytical biochemistry,
M Sugiura, and
T Oikawa, and
K Hirano
October 1980,
Clinical chemistry,
