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[Device for the high-speed cryofixation of biological matter]. 1982

A N Barnakov

A device of simple construction is proposed for rapid cryofixation of biological objects for freeze--fracture studies. The rapid freezing of the object is achieved by it immersion into the fast coolant stream, which is created in a centrifugal rotor of special construction. The freezing rate of the test-object in liquid freon-22 stream is 10 times greater compared with the ordinary cryofixation procedure using the same coolant.

UI MeSH Term Description Entries
D005614 Freeze Fracturing Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica. Fracturing, Freeze,Fracturings, Freeze,Freeze Fracturings

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